{"_buckets": {"deposit": "a6a25ba3-56d1-4fb3-91f6-1c031390c29b"}, "_deposit": {"id": "28417", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28417"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028417", "sets": ["456", "1602"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Plantlet Production by Shoot-tip Culture of a Protected Cherry-blossom Tree, Prunus lannesiana Wils. cv. Juzukake-zakura"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "38", "bibliographicPageStart": "33", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "天然記念物、梅護寺（新潟県京ヶ瀬村、（現）阿賀野市）の珠数掛桜を保存するために、組織培養法による若木の獲得を試みた。Murashige（1974）が提唱した方法に従って行った。1．無菌個体の確立のために1mg/L BA を添加したWPM 培地（Lloyd and McCown, 1980）で異なる時期に摘出した茎頂を培養したところ、9月に採取した葉芽がシュートが最もよく形成した。\\r\\\\n2．シュートを形成した培養物は0.5mg/L BA と3mg/L GA3を添加したWPM 培地に移植すると新たに複数のシュート（分枝）を形成し、シュートの生長もよかった。3．試験管内で形成されたシュートが独立栄養個体（苗）となるために必要な発根と順化法を検討した：(1)3cm 以上に伸長したシュートは発根を誘導するために５mg/L IBA を添加したWPM 培地で7日から21日間培養して発根前処理を行った；(2) 処理したシュートは、①ホルモンフリーのWPM 培地に移植する（“試験管内発根法”）か、②培土の入ったイチゴパック内で挿し木を行った（“挿し木直接発根法”）。その結果、“試験管内発根法”で得られた苗はポットに移植後の生育が不良で枯死し、“挿し木直接発根法”で得られた苗は活着がよく、培養開始から4年後には開花した。“挿し木直接発根法”の実施時期は鉢上げした苗木の管理方法を考えると6月が最適であった。", "subitem_description_type": "Abstract"}, {"subitem_description": "According to the method proposed by Murashige (1974), a system for the production of young trees by shoottip culture was developed for the Prunus lannesiana Wils. cv. Juzukake-zakura. In Stage I (Establishment of the aseptic culture), when shoot-tips were cultured on WPM media supplemented with 1 mg l-1 BA, explants excised from foliar buds in September were superior in survival and shoot formation to others excised at different times. In Stage II (Multiplication of propagula), a rapid increased number of shoots and the growth were attained in the medium with 0.5 mg l-1 BA and 3 mg l-1GA3. In Stage III (Preparation for reestablishment of plants in soil), first, about 3-cm long shoots were cultured on WPM with 5 mg l-1 IBA for 7 days to stimulate rooting, transplanted into a strawberry packing container with vermiculite and peat moss (1:1v/v), and put them in a acclimatizing room in the 14-hour light condition at 25 ℃ for 8 weeks. June was the best time to transplant rooted plants into pots, and the plants bloomed 4 years after the onset of shoot tip culture.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163470", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kasahara, Shunsaku"}]}, {"nameIdentifiers": [{"nameIdentifier": "163471", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Han, Dong-Sheng"}]}, {"nameIdentifiers": [{"nameIdentifier": "163472", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimi, Yoshiji"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "笠原, 俊策"}], "nameIdentifiers": [{"nameIdentifier": "163467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "韓, 東生"}], "nameIdentifiers": [{"nameIdentifier": "163468", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "新美, 芳二"}], "nameIdentifiers": [{"nameIdentifier": "163469", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "1_0005.pdf", "filesize": [{"value": "407.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 407600.0, "url": {"label": "1_0005.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28417/files/1_0005.pdf"}, "version_id": "a86d9a77-7578-45e4-8ef5-1db01d1049ec"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "ジュズカケザクラ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "茎頂培養", "subitem_subject_scheme": "Other"}, {"subitem_subject": "増殖", "subitem_subject_scheme": "Other"}, {"subitem_subject": "挿し木直接発根法", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prunus lannesiana Wils. cv. Juzukake-zakura", "subitem_subject_scheme": "Other"}, {"subitem_subject": "shoot-tip culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "propagation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cutting-rooting method", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得"}, {"subitem_title": "天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1602"], "permalink_uri": "http://hdl.handle.net/10191/5035", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-10-12"}, "publish_date": "2007-10-12", "publish_status": "0", "recid": "28417", "relation": {}, "relation_version_is_last": true, "title": ["天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得"], "weko_shared_id": null}