{"created":"2021-03-01T06:34:13.614084+00:00","id":28417,"links":{},"metadata":{"_buckets":{"deposit":"a6a25ba3-56d1-4fb3-91f6-1c031390c29b"},"_deposit":{"id":"28417","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28417"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028417","sets":["453:456","485:872:1583:1602"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Plantlet Production by Shoot-tip Culture of a Protected Cherry-blossom Tree, Prunus lannesiana Wils. cv. Juzukake-zakura"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"38","bibliographicPageStart":"33","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"天然記念物、梅護寺（新潟県京ヶ瀬村、（現）阿賀野市）の珠数掛桜を保存するために、組織培養法による若木の獲得を試みた。Murashige（1974）が提唱した方法に従って行った。1．無菌個体の確立のために1mg/L BA を添加したWPM 培地（Lloyd and McCown, 1980）で異なる時期に摘出した茎頂を培養したところ、9月に採取した葉芽がシュートが最もよく形成した。\\r\\\\n2．シュートを形成した培養物は0.5mg/L BA と3mg/L GA3を添加したWPM 培地に移植すると新たに複数のシュート（分枝）を形成し、シュートの生長もよかった。3．試験管内で形成されたシュートが独立栄養個体（苗）となるために必要な発根と順化法を検討した：(1)3cm 以上に伸長したシュートは発根を誘導するために５mg/L IBA を添加したWPM 培地で7日から21日間培養して発根前処理を行った；(2) 処理したシュートは、①ホルモンフリーのWPM 培地に移植する（“試験管内発根法”）か、②培土の入ったイチゴパック内で挿し木を行った（“挿し木直接発根法”）。その結果、“試験管内発根法”で得られた苗はポットに移植後の生育が不良で枯死し、“挿し木直接発根法”で得られた苗は活着がよく、培養開始から4年後には開花した。“挿し木直接発根法”の実施時期は鉢上げした苗木の管理方法を考えると6月が最適であった。","subitem_description_type":"Abstract"},{"subitem_description":"According to the method proposed by Murashige (1974), a system for the production of young trees by shoottip culture was developed for the Prunus lannesiana Wils. cv. Juzukake-zakura. In Stage I (Establishment of the aseptic culture), when shoot-tips were cultured on WPM media supplemented with 1 mg l-1 BA, explants excised from foliar buds in September were superior in survival and shoot formation to others excised at different times. In Stage II (Multiplication of propagula), a rapid increased number of shoots and the growth were attained in the medium with 0.5 mg l-1 BA and 3 mg l-1GA3. In Stage III (Preparation for reestablishment of plants in soil), first, about 3-cm long shoots were cultured on WPM with 5 mg l-1 IBA for 7 days to stimulate rooting, transplanted into a strawberry packing container with vermiculite and peat moss (1:1v/v), and put them in a acclimatizing room in the 14-hour light condition at 25 ℃ for 8 weeks. June was the best time to transplant rooted plants into pots, and the plants bloomed 4 years after the onset of shoot tip culture.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163470","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kasahara, Shunsaku"}]},{"nameIdentifiers":[{"nameIdentifier":"163471","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Han, Dong-Sheng"}]},{"nameIdentifiers":[{"nameIdentifier":"163472","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Niimi, Yoshiji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"笠原, 俊策"}],"nameIdentifiers":[{"nameIdentifier":"163467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"韓, 東生"}],"nameIdentifiers":[{"nameIdentifier":"163468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新美, 芳二"}],"nameIdentifiers":[{"nameIdentifier":"163469","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"1_0005.pdf","filesize":[{"value":"407.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1_0005.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28417/files/1_0005.pdf"},"version_id":"a86d9a77-7578-45e4-8ef5-1db01d1049ec"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ジュズカケザクラ","subitem_subject_scheme":"Other"},{"subitem_subject":"茎頂培養","subitem_subject_scheme":"Other"},{"subitem_subject":"増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"挿し木直接発根法","subitem_subject_scheme":"Other"},{"subitem_subject":"Prunus lannesiana Wils. cv. Juzukake-zakura","subitem_subject_scheme":"Other"},{"subitem_subject":"shoot-tip culture","subitem_subject_scheme":"Other"},{"subitem_subject":"propagation","subitem_subject_scheme":"Other"},{"subitem_subject":"cutting-rooting method","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得"},{"subitem_title":"天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1602"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-12"},"publish_date":"2007-10-12","publish_status":"0","recid":"28417","relation_version_is_last":true,"title":["天然記念物の梅護寺珠数掛桜（Prunus lannesiana Wils. cv. Juzukake-zakura）の茎頂培養法による増殖と若木の獲得"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:19.165431+00:00"}