{"_buckets": {"deposit": "3e9bf5b3-9cc1-416a-a054-53f9090af44c"}, "_deposit": {"id": "2000635", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2000635"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:02000635", "sets": ["455", "434"]}, "author_link": [], "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2022-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "新潟大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "Niigata University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13101", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（歯学）", "subitem_degreename_language": "ja"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objective: Food-derived bioactive peptides have been reported to exhibit various beneficial effects, including anti-microbial, anti-inflammatory, and anti-oxidant properties. Oxidative stress has been implicated in the development of several inflammatory diseases such as periodontal disease. However, the anti-oxidative effect of food-derived bioactive peptides in gingival epithelial cells (GECs) is unknown. Therefore, we examined the bioactivity of the peptides in GECs. Design: Food-derived peptide fractionations derived from rice bran, rice endosperm, corn, and soy were screened for anti-oxidative effects using anti-oxidant response element (ARE)-luciferase–transfected HEK 293 cells. The induction of anti-oxidation–related genes and proteins in GECs by the fractions were examined by quantitative PCR and Western blotting, respectively. Then, the fraction-mediated anti-oxidative effects were examined by measuring intracellular reactive oxygen species (ROS) levels using flow cytometry. Furthermore, the anti-oxidative response-related cellular signaling pathways were analyzed via Western blotting. Results: Although treatment with the food-derived peptides alone did not activate anti-oxidative responses, co-treatment with sulforaphane (SFN; a potent anti-oxidant) and certain food-derived peptides enhanced anti-oxidative responses in ARE-luciferase–transfected HEK 293 cells. The fractions augmented heme oxygenase-1 mRNA and protein expression in GECs. The percentage of ROS-positive cells was significantly decreased by co-treatment with SFN and peptide fractions derived from rice bran. Furthermore, the involvement of both nuclear factor erythroid 2-related factor 2 (Nrf2) and extracellular signal-regulated kinase (ERK) in the enhancement of anti-oxidative responses was demonstrated by Western blotting. Conclusions: Peptides derived from rice bran enhances SFN-induced anti-oxidative responses in GECs through ERK–Nrf2–ARE signaling.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Archives of Oral Biology. 2021, 129, 105215.", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博(歯)第505号", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第5019号"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.archoralbio.2021.105215", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2021 Elsevier Ltd. All rights reserved.", "subitem_rights_language": "en"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mineo, Shuhei", "creatorNameLang": "en"}, {"creatorName": "峯尾, 修平", "creatorNameLang": "ja"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"date": [{"dateType": "Available", "dateValue": "2022-07-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r3ndk505.pdf", "filesize": [{"value": "2.84MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": "2.84MB", "url": {"label": "本文", "objectType": "fulltext", "url": "https://niigata-u.repo.nii.ac.jp/record/2000635/files/r3ndk505.pdf"}, "version_id": "abf2af03-3b13-4b26-ba7d-218e39cff51b"}, {"date": [{"dateType": "Available", "dateValue": "2022-07-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "r3ndk505_a.pdf", "filesize": [{"value": "391KB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": "391KB", "url": {"label": "要旨", "objectType": "abstract", "url": "https://niigata-u.repo.nii.ac.jp/record/2000635/files/r3ndk505_a.pdf"}, "version_id": "1053711c-8873-4b57-9261-12039f639d58"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Gingival epithelial cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Periodontitis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oxidative stress", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells", "subitem_title_language": "en"}, {"subitem_title": "米糠由来のタンパク分画は歯肉上皮細胞におけるSFN誘導性の抗酸化活性を増強させる", "subitem_title_language": "ja"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "434"], "permalink_uri": "http://hdl.handle.net/10191/0002000635", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-07-25"}, "publish_date": "2022-07-25", "publish_status": "0", "recid": "2000635", "relation": {}, "relation_version_is_last": true, "title": ["Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells"], "weko_shared_id": -1}