@phdthesis{oai:niigata-u.repo.nii.ac.jp:02000635,
 author = {Mineo, Shuhei and 峯尾, 修平},
 month = {2022-07-25, 2022-07-25},
 note = {Objective: Food-derived bioactive peptides have been reported to exhibit various beneficial effects, including anti-microbial, anti-inflammatory, and anti-oxidant properties. Oxidative stress has been implicated in the development of several inflammatory diseases such as periodontal disease. However, the anti-oxidative effect of food-derived bioactive peptides in gingival epithelial cells (GECs) is unknown. Therefore, we examined the bioactivity of the peptides in GECs. Design: Food-derived peptide fractionations derived from rice bran, rice endosperm, corn, and soy were screened for anti-oxidative effects using anti-oxidant response element (ARE)-luciferase–transfected HEK 293 cells. The induction of anti-oxidation–related genes and proteins in GECs by the fractions were examined by quantitative PCR and Western blotting, respectively. Then, the fraction-mediated anti-oxidative effects were examined by measuring intracellular reactive oxygen species (ROS) levels using flow cytometry. Furthermore, the anti-oxidative response-related cellular signaling pathways were analyzed via Western blotting. Results: Although treatment with the food-derived peptides alone did not activate anti-oxidative responses, co-treatment with sulforaphane (SFN; a potent anti-oxidant) and certain food-derived peptides enhanced anti-oxidative responses in ARE-luciferase–transfected HEK 293 cells. The fractions augmented heme oxygenase-1 mRNA and protein expression in GECs. The percentage of ROS-positive cells was significantly decreased by co-treatment with SFN and peptide fractions derived from rice bran. Furthermore, the involvement of both nuclear factor erythroid 2-related factor 2 (Nrf2) and extracellular signal-regulated kinase (ERK) in the enhancement of anti-oxidative responses was demonstrated by Western blotting. Conclusions: Peptides derived from rice bran enhances SFN-induced anti-oxidative responses in GECs through ERK–Nrf2–ARE signaling., Archives of Oral Biology. 2021, 129, 105215., 新大院博(歯)第505号},
 school = {新潟大学, Niigata University},
 title = {Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells},
 year = {}
}