{"created":"2022-07-25T06:27:23.482473+00:00","id":2000635,"links":{},"metadata":{"_buckets":{"deposit":"3e9bf5b3-9cc1-416a-a054-53f9090af44c"},"_deposit":{"id":"2000635","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000635"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000635","sets":["432:433:434","453:455"]},"author_link":[],"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Food-derived bioactive peptides have been reported to exhibit various beneficial effects, including anti-microbial, anti-inflammatory, and anti-oxidant properties. Oxidative stress has been implicated in the development of several inflammatory diseases such as periodontal disease. However, the anti-oxidative effect of food-derived bioactive peptides in gingival epithelial cells (GECs) is unknown. Therefore, we examined the bioactivity of the peptides in GECs. Design: Food-derived peptide fractionations derived from rice bran, rice endosperm, corn, and soy were screened for anti-oxidative effects using anti-oxidant response element (ARE)-luciferase–transfected HEK 293 cells. The induction of anti-oxidation–related genes and proteins in GECs by the fractions were examined by quantitative PCR and Western blotting, respectively. Then, the fraction-mediated anti-oxidative effects were examined by measuring intracellular reactive oxygen species (ROS) levels using flow cytometry. Furthermore, the anti-oxidative response-related cellular signaling pathways were analyzed via Western blotting. Results: Although treatment with the food-derived peptides alone did not activate anti-oxidative responses, co-treatment with sulforaphane (SFN; a potent anti-oxidant) and certain food-derived peptides enhanced anti-oxidative responses in ARE-luciferase–transfected HEK 293 cells. The fractions augmented heme oxygenase-1 mRNA and protein expression in GECs. The percentage of ROS-positive cells was significantly decreased by co-treatment with SFN and peptide fractions derived from rice bran. Furthermore, the involvement of both nuclear factor erythroid 2-related factor 2 (Nrf2) and extracellular signal-regulated kinase (ERK) in the enhancement of anti-oxidative responses was demonstrated by Western blotting. Conclusions: Peptides derived from rice bran enhances SFN-induced anti-oxidative responses in GECs through ERK–Nrf2–ARE signaling.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Archives of Oral Biology. 2021, 129, 105215.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(歯)第505号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第5019号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.archoralbio.2021.105215","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 Elsevier Ltd. All rights reserved.","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mineo, Shuhei","creatorNameLang":"en"},{"creatorName":"峯尾, 修平","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-07-25"}],"displaytype":"detail","filename":"r3ndk505.pdf","filesize":[{"value":"2.84MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000635/files/r3ndk505.pdf"},"version_id":"abf2af03-3b13-4b26-ba7d-218e39cff51b"},{"date":[{"dateType":"Available","dateValue":"2022-07-25"}],"displaytype":"detail","filename":"r3ndk505_a.pdf","filesize":[{"value":"391KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000635/files/r3ndk505_a.pdf"},"version_id":"1053711c-8873-4b57-9261-12039f639d58"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Gingival epithelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Periodontitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oxidative stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells","subitem_title_language":"en"},{"subitem_title":"米糠由来のタンパク分画は歯肉上皮細胞におけるSFN誘導性の抗酸化活性を増強させる","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-25"},"publish_date":"2022-07-25","publish_status":"0","recid":"2000635","relation_version_is_last":true,"title":["Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:18:00.526316+00:00"}