{"_buckets": {"deposit": "6a0bbf6a-0c88-4181-a3ed-3b7e0da230ef"}, "_deposit": {"id": "8748", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "8748"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00008748", "sets": ["456", "913"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Induction of Antigen Specific Cytotoxic T Lymphocytes (CTLs) by Plasmacytoid Leukemic Cell Line (PMDC11) or PMDC11-derived Exosomes"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "139", "bibliographicPageStart": "125", "bibliographicVolumeNumber": "129", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "私たちは白血病性形質細胞様樹状細胞株(PMDC05)を樹立し, この細胞株が抗原特異的細胞傷害性T細胞(CTL)誘導能を有することを報告してきた. さらに, CD80遺伝子を導入したPMDC05であるPMDC11の抗原提示能がさらに増強することも報告した. エクソソームは, 多くの細胞により分泌される30～100nmの大きさの小粒子であり, 抗原提示細胞(APC)由来エクソソームは主要組織適合抗原(MHC)や抗原提示関連分子などを多く含んでいることが知られており, 単球由来樹状細胞(moDC)の分泌するエクソソーム(dexosome)が抗原提示能を有することが示され, これを用いた臨床研究も行われている. 本研究では, PMDC11細胞由来エクソソームを同定するとともに, PMDC11細胞とそのエクソソームによるCTL誘導について検討した. 抗原ペプチドをパルスしたPMDC11細胞またはmoDCの培養上清からExoQuick-TCを用いてエクソソームを抽出した. この抽出物がエクソソームであることを, 磁気ビーズとフローサイトメトリーを用いて確認した. 磁気ビーズで捕捉した抽出物は, HLA-DR, CD54, CD86, CD80, CD123陽性であり, さらにエクソソームマーカーであるCD63(LAMP3)が陽性であることから, PMDC11細胞はエクソソーム(PMDC11-dex)を放出することが確認された. 次に, サイトメガロウイルス(CMV)pp65ペプチドまたは改変型ウィルムス腫瘍遺伝子(mWT1)ペプチドをパルスしたPMDC11細胞を用いてHLA-A*24:02健常人末梢血CD8+T細胞を刺激培養し, 抗原特異的CTL誘導についてテトラマー解析を行った. PMDC11細胞刺激培養において, 抗原特異的CTLの増幅が確認された. CMV pp65ペプチドまたはmWT1ペプチドをパルスしたPMDC11細胞培養上清より抽出したPMDC11-dexによる刺激培養では, PMDC11-dex添加培養系において抗原特異的CTLの増幅が確認された. これらの結果よりPMDC細胞株はエクソソーム分泌能有し, PMDC11-dexは抗原特異的CTL誘導能を有することが示唆された. PMDC11細胞は, 細胞自体がCTL養子免疫療法に応用できるのみならず, エクソソームを用いた無細胞系ワクチンの開発にも有用であると思われた.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "61003", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Uchiyama, Takayoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "61004", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Narita, Miwako"}]}, {"nameIdentifiers": [{"nameIdentifier": "61005", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwaya, Shunpei"}]}, {"nameIdentifiers": [{"nameIdentifier": "61006", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Oiwa, Eri"}]}, {"nameIdentifiers": [{"nameIdentifier": "61007", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nishizawa, Yoshinori"}]}, {"nameIdentifiers": [{"nameIdentifier": "61008", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Takahashi, Masuhiro"}]}, {"nameIdentifiers": [{"nameIdentifier": "61009", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Hashimoto, Shigeo"}]}, {"nameIdentifiers": [{"nameIdentifier": "61010", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Takizawa, Jun"}]}, {"nameIdentifiers": [{"nameIdentifier": "61011", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Sone, Hirohito"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "内山, 孝由"}], "nameIdentifiers": [{"nameIdentifier": "60993", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "成田, 美和子"}], "nameIdentifiers": [{"nameIdentifier": "60994", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩谷, 俊平"}], "nameIdentifiers": [{"nameIdentifier": "60995", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大岩, 恵理"}], "nameIdentifiers": [{"nameIdentifier": "60996", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "西澤, 幹則"}], "nameIdentifiers": [{"nameIdentifier": "60997", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋, 益廣"}], "nameIdentifiers": [{"nameIdentifier": "60998", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "橋本, 誠雄"}], "nameIdentifiers": [{"nameIdentifier": "60999", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "瀧澤, 淳"}], "nameIdentifiers": [{"nameIdentifier": "61000", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "曽根, 博仁"}], "nameIdentifiers": [{"nameIdentifier": "61001", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kasahara, Noriyuki"}], "nameIdentifiers": [{"nameIdentifier": "61002", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "129(3)_125-139.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "129(3)_125-139.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/8748/files/129(3)_125-139.pdf"}, "version_id": "cb53ebda-b495-467d-a64b-2e59ce4159d7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "白血病性形質細胞様樹状細胞株", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PMDC11", "subitem_subject_scheme": "Other"}, {"subitem_subject": "エクソソーム", "subitem_subject_scheme": "Other"}, {"subitem_subject": "サイトメガロウィルス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "WT1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞傷害性T細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "テトラマー解析", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導"}, {"subitem_title": "白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "913"], "permalink_uri": "http://hdl.handle.net/10191/44037", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-09-27"}, "publish_date": "2016-09-27", "publish_status": "0", "recid": "8748", "relation": {}, "relation_version_is_last": true, "title": ["白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導"], "weko_shared_id": null}