{"created":"2021-03-01T06:12:46.967316+00:00","id":8748,"links":{},"metadata":{"_buckets":{"deposit":"6a0bbf6a-0c88-4181-a3ed-3b7e0da230ef"},"_deposit":{"id":"8748","owners":[],"pid":{"revision_id":0,"type":"depid","value":"8748"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00008748","sets":["453:456","471:537:538:913"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Induction of Antigen Specific Cytotoxic T Lymphocytes (CTLs) by Plasmacytoid Leukemic Cell Line (PMDC11) or PMDC11-derived Exosomes"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"139","bibliographicPageStart":"125","bibliographicVolumeNumber":"129","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"私たちは白血病性形質細胞様樹状細胞株(PMDC05)を樹立し, この細胞株が抗原特異的細胞傷害性T細胞(CTL)誘導能を有することを報告してきた. さらに, CD80遺伝子を導入したPMDC05であるPMDC11の抗原提示能がさらに増強することも報告した. エクソソームは, 多くの細胞により分泌される30～100nmの大きさの小粒子であり, 抗原提示細胞(APC)由来エクソソームは主要組織適合抗原(MHC)や抗原提示関連分子などを多く含んでいることが知られており, 単球由来樹状細胞(moDC)の分泌するエクソソーム(dexosome)が抗原提示能を有することが示され, これを用いた臨床研究も行われている. 本研究では, PMDC11細胞由来エクソソームを同定するとともに, PMDC11細胞とそのエクソソームによるCTL誘導について検討した. 抗原ペプチドをパルスしたPMDC11細胞またはmoDCの培養上清からExoQuick-TCを用いてエクソソームを抽出した. この抽出物がエクソソームであることを, 磁気ビーズとフローサイトメトリーを用いて確認した. 磁気ビーズで捕捉した抽出物は, HLA-DR, CD54, CD86, CD80, CD123陽性であり, さらにエクソソームマーカーであるCD63(LAMP3)が陽性であることから, PMDC11細胞はエクソソーム(PMDC11-dex)を放出することが確認された. 次に, サイトメガロウイルス(CMV)pp65ペプチドまたは改変型ウィルムス腫瘍遺伝子(mWT1)ペプチドをパルスしたPMDC11細胞を用いてHLA-A*24:02健常人末梢血CD8+T細胞を刺激培養し, 抗原特異的CTL誘導についてテトラマー解析を行った. PMDC11細胞刺激培養において, 抗原特異的CTLの増幅が確認された. CMV pp65ペプチドまたはmWT1ペプチドをパルスしたPMDC11細胞培養上清より抽出したPMDC11-dexによる刺激培養では, PMDC11-dex添加培養系において抗原特異的CTLの増幅が確認された. これらの結果よりPMDC細胞株はエクソソーム分泌能有し, PMDC11-dexは抗原特異的CTL誘導能を有することが示唆された. PMDC11細胞は, 細胞自体がCTL養子免疫療法に応用できるのみならず, エクソソームを用いた無細胞系ワクチンの開発にも有用であると思われた.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"61003","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Uchiyama, Takayoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"61004","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Miwako"}]},{"nameIdentifiers":[{"nameIdentifier":"61005","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwaya, Shunpei"}]},{"nameIdentifiers":[{"nameIdentifier":"61006","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Oiwa, Eri"}]},{"nameIdentifiers":[{"nameIdentifier":"61007","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nishizawa, Yoshinori"}]},{"nameIdentifiers":[{"nameIdentifier":"61008","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masuhiro"}]},{"nameIdentifiers":[{"nameIdentifier":"61009","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hashimoto, Shigeo"}]},{"nameIdentifiers":[{"nameIdentifier":"61010","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takizawa, Jun"}]},{"nameIdentifiers":[{"nameIdentifier":"61011","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sone, Hirohito"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"内山, 孝由"}],"nameIdentifiers":[{"nameIdentifier":"60993","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"成田, 美和子"}],"nameIdentifiers":[{"nameIdentifier":"60994","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩谷, 俊平"}],"nameIdentifiers":[{"nameIdentifier":"60995","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大岩, 恵理"}],"nameIdentifiers":[{"nameIdentifier":"60996","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西澤, 幹則"}],"nameIdentifiers":[{"nameIdentifier":"60997","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 益廣"}],"nameIdentifiers":[{"nameIdentifier":"60998","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"橋本, 誠雄"}],"nameIdentifiers":[{"nameIdentifier":"60999","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧澤, 淳"}],"nameIdentifiers":[{"nameIdentifier":"61000","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽根, 博仁"}],"nameIdentifiers":[{"nameIdentifier":"61001","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kasahara, Noriyuki"}],"nameIdentifiers":[{"nameIdentifier":"61002","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"129(3)_125-139.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"129(3)_125-139.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/8748/files/129(3)_125-139.pdf"},"version_id":"cb53ebda-b495-467d-a64b-2e59ce4159d7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"白血病性形質細胞様樹状細胞株","subitem_subject_scheme":"Other"},{"subitem_subject":"PMDC11","subitem_subject_scheme":"Other"},{"subitem_subject":"エクソソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトメガロウィルス","subitem_subject_scheme":"Other"},{"subitem_subject":"WT1","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害性T細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"テトラマー解析","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導"},{"subitem_title":"白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","913"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-09-27"},"publish_date":"2016-09-27","publish_status":"0","recid":"8748","relation_version_is_last":true,"title":["白血病性形質細胞様樹状細胞株 (PMDC11) およびPMDC11由来エクソソームを用いた抗原特異的細胞傷害性T細胞 (CTL) の誘導"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:43:03.246029+00:00"}