{"_buckets": {"deposit": "f9bccc84-c848-470a-bd45-9e4467b4392c"}, "_deposit": {"id": "6483", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6483"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006483"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1995-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "57", "bibliographicPageStart": "53", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5\u00b112.4% vs 85.1%\u00b15.9%, p\u003c0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3\u00b17.6% vs 85.1\u00b15.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Itoi, Takao"}], "nameIdentifiers": [{"nameIdentifier": "53140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Hidenobu"}], "nameIdentifiers": [{"nameIdentifier": "53141", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshida, Mitsuhiro"}], "nameIdentifiers": [{"nameIdentifier": "53142", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kasuya, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "53143", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokota, Yoko"}], "nameIdentifiers": [{"nameIdentifier": "53144", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "43(1)_53-57.pdf", "filesize": [{"value": "738.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 738500.0, "url": {"label": "43(1)_53-57.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6483/files/43(1)_53-57.pdf"}, "version_id": "69f7dcde-8315-46a2-a956-314e647b40f2"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies"}, {"subitem_title": "p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/624"], "permalink_uri": "http://hdl.handle.net/10191/33197", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-08-31"}, "publish_date": "2015-08-31", "publish_status": "0", "recid": "6483", "relation": {}, "relation_version_is_last": true, "title": ["p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies"], "weko_shared_id": null}