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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.43 No.1

p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies

http://hdl.handle.net/10191/33197
http://hdl.handle.net/10191/33197
86ecd96a-5c95-48a0-8658-b849235c61c4
名前 / ファイル ライセンス アクション
43(1)_53-57.pdf 43(1)_53-57.pdf (738.5 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-31
タイトル
タイトル p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies
タイトル
言語 en
タイトル p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Itoi, Takao

× Itoi, Takao

WEKO 53140

Itoi, Takao

Search repository
Watanabe, Hidenobu

× Watanabe, Hidenobu

WEKO 53141

Watanabe, Hidenobu

Search repository
Yoshida, Mitsuhiro

× Yoshida, Mitsuhiro

WEKO 53142

Yoshida, Mitsuhiro

Search repository
Kasuya, Kazuhiko

× Kasuya, Kazuhiko

WEKO 53143

Kasuya, Kazuhiko

Search repository
Yokota, Yoko

× Yokota, Yoko

WEKO 53144

Yokota, Yoko

Search repository
抄録
内容記述タイプ Abstract
内容記述 p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5±12.4% vs 85.1%±5.9%, p<0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3±7.6% vs 85.1±5.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 43, 号 1, p. 53-57, 発行日 1995-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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