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p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies
http://hdl.handle.net/10191/33197
http://hdl.handle.net/10191/3319786ecd96a-5c95-48a0-8658-b849235c61c4
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2015-08-31 | |||||
タイトル | ||||||
タイトル | p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies | |||||
言語 | ||||||
言語 | eng | |||||
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資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
Itoi, Takao
× Itoi, Takao× Watanabe, Hidenobu× Yoshida, Mitsuhiro× Kasuya, Kazuhiko× Yokota, Yoko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5±12.4% vs 85.1%±5.9%, p<0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3±7.6% vs 85.1±5.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used. | |||||
書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 43, 号 1, p. 53-57, 発行日 1995-03 |
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出版者 | Niigata University School of Medicine | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05677734 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508361 | |||||
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値 | publisher |