{"created":"2021-03-01T06:10:23.529079+00:00","id":6483,"links":{},"metadata":{"_buckets":{"deposit":"f9bccc84-c848-470a-bd45-9e4467b4392c"},"_deposit":{"id":"6483","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6483"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006483","sets":["453:456","471:537:568:624"]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"57","bibliographicPageStart":"53","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Acta medica et biologica"},{"bibliographic_title":"Acta medica et biologica","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5±12.4% vs 85.1%±5.9%, p<0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3±7.6% vs 85.1±5.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Niigata University School of Medicine"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508361","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05677734","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Itoi, Takao"}],"nameIdentifiers":[{"nameIdentifier":"53140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Hidenobu"}],"nameIdentifiers":[{"nameIdentifier":"53141","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Mitsuhiro"}],"nameIdentifiers":[{"nameIdentifier":"53142","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kasuya, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"53143","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokota, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"53144","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"43(1)_53-57.pdf","filesize":[{"value":"738.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"43(1)_53-57.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/6483/files/43(1)_53-57.pdf"},"version_id":"69f7dcde-8315-46a2-a956-314e647b40f2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies"},{"subitem_title":"p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","624"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-08-31"},"publish_date":"2015-08-31","publish_status":"0","recid":"6483","relation_version_is_last":true,"title":["p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:39:46.783352+00:00"}