@article{oai:niigata-u.repo.nii.ac.jp:00006483,
 author = {Itoi, Takao and Watanabe, Hidenobu and Yoshida, Mitsuhiro and Kasuya, Kazuhiko and Yokota, Yoko},
 issue = {1},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Mar},
 note = {p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5±12.4% vs 85.1%±5.9%, p<0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3±7.6% vs 85.1±5.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used.},
 pages = {53--57},
 title = {p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies},
 volume = {43},
 year = {1995}
}