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Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression
http://hdl.handle.net/10191/50430
http://hdl.handle.net/10191/504306362b43c-79dd-4073-8829-4a9169efd81e
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本文 (4.7 MB)
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要旨 (603.1 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2018-07-26 | |||||
| タイトル | ||||||
| タイトル | Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression | |||||
| タイトル | ||||||
| タイトル | Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | NS-GFP導入遺伝子の発現量に基づく幹細胞未分化性モニタリングシステムで機能的な造血幹細胞集団を分離する | |||||
| 著者 |
Fuse, Kyoko
× Fuse, Kyoko |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 51231 | |||||
| 姓名 | 布施, 香子 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hematopoietic stem cells (HSCs) in a steady state can be efficiently purified by selecting for a combination of several cell surface markers; however, such markers do not consistently reflect HSC activity. In this study, we successfully enriched HSCs with a unique stemness-monitoring system using a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene. We found that the phenotypically defined long-term (LT)-HSC population exhibited the highest level of NS-GFP intensity, whereas NS-GFP intensity was strongly downregulated during differentiation in vitro and in vivo. Within the LT-HSC population, NS-GFP^<high> cells exhibited significantly higher repopulating capacity than NS-GFP^<low> cells. Gene expression analysis revealed that nine genes, including Vwf and Cdkn1c (p57), are highly expressed in NS-GFP^<high> cells and may represent a signature of HSCs, i.e., a stemness signature. When LT-HSCs suffered from remarkable stress, such as transplantation or irradiation, NS-GFP intensity was downregulated. Finally, we found that high levels of NS-GFP identified HSC-like cells even among CD34^+ cells, which have been considered progenitor cells without long-term reconstitution ability. Thus, high NS-GFP expression represents stem cell characteristics in hematopoietic cells, making this system useful for identifying previously uncharacterized HSCs. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 甲第4404号. 学位記番号: 新大院博（医）甲第803号. 学位授与年月日: 平成30年3月23日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Scientific Reports 7: 11442 | |||||
| 書誌情報 | 発行日 2018-03-23 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1038/s41598-017-11909-3 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4404号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（医）甲第803号 | |||||
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Cite as
Fuse, Kyoko, 2018, Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression: 新潟大学.
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