{"_buckets": {"deposit": "b3b478d4-9e03-4080-8ba7-9f75056717e0"}, "_deposit": {"id": "6002", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6002"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006002"}, "item_6_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "NS-GFP\u5c0e\u5165\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u306b\u57fa\u3065\u304f\u5e79\u7d30\u80de\u672a\u5206\u5316\u6027\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0\u3067\u6a5f\u80fd\u7684\u306a\u9020\u8840\u5e79\u7d30\u80de\u96c6\u56e3\u3092\u5206\u96e2\u3059\u308b"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-03-23", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_6_date_granted_51": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2018-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "\u65b0\u6f5f\u5927\u5b66"}]}]}, "item_6_degree_name_48": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb\uff08\u533b\u5b66\uff09"}]}, "item_6_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Hematopoietic stem cells (HSCs) in a steady state can be efficiently purified by selecting for a combination of several cell surface markers; however, such markers do not consistently reflect HSC activity. In this study, we successfully enriched HSCs with a unique stemness-monitoring system using a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene. We found that the phenotypically defined long-term (LT)-HSC population exhibited the highest level of NS-GFP intensity, whereas NS-GFP intensity was strongly downregulated during differentiation in vitro and in vivo. Within the LT-HSC population, NS-GFP^\u003chigh\u003e cells exhibited significantly higher repopulating capacity than NS-GFP^\u003clow\u003e cells. Gene expression analysis revealed that nine genes, including Vwf and Cdkn1c (p57), are highly expressed in NS-GFP^\u003chigh\u003e cells and may represent a signature of HSCs, i.e., a stemness signature. When LT-HSCs suffered from remarkable stress, such as transplantation or irradiation, NS-GFP intensity was downregulated. Finally, we found that high levels of NS-GFP identified HSC-like cells even among CD34^+ cells, which have been considered progenitor cells without long-term reconstitution ability. Thus, high NS-GFP expression represents stem cell characteristics in hematopoietic cells, making this system useful for identifying previously uncharacterized HSCs.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u5b66\u4f4d\u306e\u7a2e\u985e: \u535a\u58eb\uff08\u533b\u5b66\uff09. \u5831\u544a\u756a\u53f7: \u7532\u7b2c4404\u53f7. \u5b66\u4f4d\u8a18\u756a\u53f7: \u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c803\u53f7. \u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5: \u5e73\u621030\u5e743\u670823\u65e5", "subitem_description_type": "Other"}, {"subitem_description": "Scientific Reports 7: 11442", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "\u5b66\u4f4d\u8a18\u756a\u53f7", "attribute_value_mlt": [{"subitem_description": "\u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c803\u53f7", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101\u7532\u7b2c4404\u53f7"}]}, "item_6_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "51231", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5e03\u65bd, \u9999\u5b50"}]}]}, "item_6_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1038/s41598-017-11909-3", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fuse, Kyoko"}], "nameIdentifiers": [{"nameIdentifier": "51230", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "h29nmk803.pdf", "filesize": [{"value": "4.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 4700000.0, "url": {"label": "\u672c\u6587", "url": "https://niigata-u.repo.nii.ac.jp/record/6002/files/h29nmk803.pdf"}, "version_id": "f1b9c653-ac59-48ac-a2ab-ee2f1fb07e4d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "h29nmk803_a.pdf", "filesize": [{"value": "603.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 603100.0, "url": {"label": "\u8981\u65e8", "url": "https://niigata-u.repo.nii.ac.jp/record/6002/files/h29nmk803_a.pdf"}, "version_id": "bd805751-d3f7-4b88-92fa-ca459b9186cb"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression"}, {"subitem_title": "Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["453/455", "471/561/562"], "permalink_uri": "http://hdl.handle.net/10191/50430", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2018-07-26"}, "publish_date": "2018-07-26", "publish_status": "0", "recid": "6002", "relation": {}, "relation_version_is_last": true, "title": ["Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression"], "weko_shared_id": 2}