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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression

http://hdl.handle.net/10191/50430
http://hdl.handle.net/10191/50430
6362b43c-79dd-4073-8829-4a9169efd81e
名前 / ファイル ライセンス アクション
h29nmk803.pdf 本文 (4.7 MB)
h29nmk803_a.pdf 要旨 (603.1 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2018-07-26
タイトル
タイトル Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression
タイトル
言語 en
タイトル Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル NS-GFP導入遺伝子の発現量に基づく幹細胞未分化性モニタリングシステムで機能的な造血幹細胞集団を分離する
著者 Fuse, Kyoko

× Fuse, Kyoko

WEKO 51230

Fuse, Kyoko

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著者別名
識別子 51231
識別子Scheme WEKO
姓名 布施, 香子
抄録
内容記述タイプ Abstract
内容記述 Hematopoietic stem cells (HSCs) in a steady state can be efficiently purified by selecting for a combination of several cell surface markers; however, such markers do not consistently reflect HSC activity. In this study, we successfully enriched HSCs with a unique stemness-monitoring system using a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene. We found that the phenotypically defined long-term (LT)-HSC population exhibited the highest level of NS-GFP intensity, whereas NS-GFP intensity was strongly downregulated during differentiation in vitro and in vivo. Within the LT-HSC population, NS-GFP^<high> cells exhibited significantly higher repopulating capacity than NS-GFP^<low> cells. Gene expression analysis revealed that nine genes, including Vwf and Cdkn1c (p57), are highly expressed in NS-GFP^<high> cells and may represent a signature of HSCs, i.e., a stemness signature. When LT-HSCs suffered from remarkable stress, such as transplantation or irradiation, NS-GFP intensity was downregulated. Finally, we found that high levels of NS-GFP identified HSC-like cells even among CD34^+ cells, which have been considered progenitor cells without long-term reconstitution ability. Thus, high NS-GFP expression represents stem cell characteristics in hematopoietic cells, making this system useful for identifying previously uncharacterized HSCs.
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第4404号. 学位記番号: 新大院博(医)甲第803号. 学位授与年月日: 平成30年3月23日
内容記述
内容記述タイプ Other
内容記述 Scientific Reports 7: 11442
書誌情報 発行日 2018-03-23
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1038/s41598-017-11909-3
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2018-03-23
学位授与番号
学位授与番号 13101甲第4404号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第803号
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