{"created":"2021-03-01T06:09:53.211215+00:00","id":6002,"links":{},"metadata":{"_buckets":{"deposit":"b3b478d4-9e03-4080-8ba7-9f75056717e0"},"_deposit":{"id":"6002","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6002"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006002"},"item_6_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"NS-GFP\u5c0e\u5165\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u306b\u57fa\u3065\u304f\u5e79\u7d30\u80de\u672a\u5206\u5316\u6027\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0\u3067\u6a5f\u80fd\u7684\u306a\u9020\u8840\u5e79\u7d30\u80de\u96c6\u56e3\u3092\u5206\u96e2\u3059\u308b"}]},"item_6_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5","attribute_value_mlt":[{"subitem_dategranted":"2018-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"\u65b0\u6f5f\u5927\u5b66"}]}]},"item_6_degree_name_48":{"attribute_name":"\u5b66\u4f4d\u540d","attribute_value_mlt":[{"subitem_degreename":"\u535a\u58eb\uff08\u533b\u5b66\uff09"}]},"item_6_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"Hematopoietic stem cells (HSCs) in a steady state can be efficiently purified by selecting for a combination of several cell surface markers; however, such markers do not consistently reflect HSC activity. In this study, we successfully enriched HSCs with a unique stemness-monitoring system using a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene. We found that the phenotypically defined long-term (LT)-HSC population exhibited the highest level of NS-GFP intensity, whereas NS-GFP intensity was strongly downregulated during differentiation in vitro and in vivo. Within the LT-HSC population, NS-GFP^<high> cells exhibited significantly higher repopulating capacity than NS-GFP^<low> cells. Gene expression analysis revealed that nine genes, including Vwf and Cdkn1c (p57), are highly expressed in NS-GFP^<high> cells and may represent a signature of HSCs, i.e., a stemness signature. When LT-HSCs suffered from remarkable stress, such as transplantation or irradiation, NS-GFP intensity was downregulated. Finally, we found that high levels of NS-GFP identified HSC-like cells even among CD34^+ cells, which have been considered progenitor cells without long-term reconstitution ability. Thus, high NS-GFP expression represents stem cell characteristics in hematopoietic cells, making this system useful for identifying previously uncharacterized HSCs.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"\u5185\u5bb9\u8a18\u8ff0","attribute_value_mlt":[{"subitem_description":"\u5b66\u4f4d\u306e\u7a2e\u985e: \u535a\u58eb\uff08\u533b\u5b66\uff09. \u5831\u544a\u756a\u53f7: \u7532\u7b2c4404\u53f7. \u5b66\u4f4d\u8a18\u756a\u53f7: \u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c803\u53f7. \u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5: \u5e73\u621030\u5e743\u670823\u65e5","subitem_description_type":"Other"},{"subitem_description":"Scientific Reports 7: 11442","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"\u5b66\u4f4d\u8a18\u756a\u53f7","attribute_value_mlt":[{"subitem_description":"\u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c803\u53f7","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7","attribute_value_mlt":[{"subitem_dissertationnumber":"13101\u7532\u7b2c4404\u53f7"}]},"item_6_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"51231","nameIdentifierScheme":"WEKO"}],"names":[{"name":"\u5e03\u65bd, \u9999\u5b50"}]}]},"item_6_publisher_7":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"\u65b0\u6f5f\u5927\u5b66"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-017-11909-3","subitem_relation_type_select":"DOI"}}]},"item_6_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fuse, Kyoko"}],"nameIdentifiers":[{"nameIdentifier":"51230","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h29nmk803.pdf","filesize":[{"value":"4.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"\u672c\u6587","url":"https://niigata-u.repo.nii.ac.jp/record/6002/files/h29nmk803.pdf"},"version_id":"f1b9c653-ac59-48ac-a2ab-ee2f1fb07e4d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h29nmk803_a.pdf","filesize":[{"value":"603.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"\u8981\u65e8","url":"https://niigata-u.repo.nii.ac.jp/record/6002/files/h29nmk803_a.pdf"},"version_id":"bd805751-d3f7-4b88-92fa-ca459b9186cb"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression"},{"subitem_title":"Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["453/455","471/561/562"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2018-07-26"},"publish_date":"2018-07-26","publish_status":"0","recid":"6002","relation_version_is_last":true,"title":["Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2021-03-01T08:39:49.084047+00:00"}