@misc{oai:niigata-u.repo.nii.ac.jp:00006002,
 author = {Fuse, Kyoko},
 month = {Mar},
 note = {Hematopoietic stem cells (HSCs) in a steady state can be efficiently purified by selecting for a combination of several cell surface markers; however, such markers do not consistently reflect HSC activity. In this study, we successfully enriched HSCs with a unique stemness-monitoring system using a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene. We found that the phenotypically defined long-term (LT)-HSC population exhibited the highest level of NS-GFP intensity, whereas NS-GFP intensity was strongly downregulated during differentiation in vitro and in vivo. Within the LT-HSC population, NS-GFP^<high> cells exhibited significantly higher repopulating capacity than NS-GFP^<low> cells. Gene expression analysis revealed that nine genes, including Vwf and Cdkn1c (p57), are highly expressed in NS-GFP^<high> cells and may represent a signature of HSCs, i.e., a stemness signature. When LT-HSCs suffered from remarkable stress, such as transplantation or irradiation, NS-GFP intensity was downregulated. Finally, we found that high levels of NS-GFP identified HSC-like cells even among CD34^+ cells, which have been considered progenitor cells without long-term reconstitution ability. Thus, high NS-GFP expression represents stem cell characteristics in hematopoietic cells, making this system useful for identifying previously uncharacterized HSCs., 学位の種類: 博士（医学）. 報告番号: 甲第4404号. 学位記番号: 新大院博（医）甲第803号. 学位授与年月日: 平成30年3月23日, Scientific Reports 7: 11442, 新大院博（医）甲第803号},
 title = {Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression},
 year = {2018}
}