{"_buckets": {"deposit": "92f1c469-5fe3-4375-94b4-4feb072ba774"}, "_deposit": {"id": "28578", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28578"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028578", "sets": ["456", "1630"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Identification of IFN-γ production and cytotoxicity in WT1 tetramer+ T cells generated by mixed lymphocyte peptide culture in WT1 peptide immunized persons"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "65", "bibliographicPageStart": "57", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "新潟大学保健学雑誌"}, {"bibliographic_title": "新潟大学保健学雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "抗腫瘍免疫療法施行例における治療効果を判定するために，in vitroで培養された腫瘍抗原特異的細胞傷害性T細胞（CTL）が特異的に抗原を認識し，細胞傷害機能を有するか調べることを目的とした。本検討では，in vivoに存在するWT1特異的CTLを混合リンパ球ペプチド培養（mixed lymphocyte peptide culture; MLPC）によりin vitroで増幅し，WT1特異的CTLの機能解析を行った。in vitroで誘導されたWT1特異的CTLはWT1抗原特異的にIFN-γを合成し，細胞傷害活性を有することが確認された。以上のことから，WT1ペプチドワクチン施行例の末梢血中にはIFN-γの産生能と細胞傷害活性を持つWT1特異的CTLが存在し，これらのCTLはさらなるWT1ペプチドワクチンにより増幅されると考えられた。", "subitem_description_type": "Abstract"}, {"subitem_description": "Although the frequencies of cytotoxic T lymphocytes（CTLs）in peripheral blood（PB）can be evaluated by mixed lymphocyte peptide culture（MLPC）, the function of tetramer+ CD8+ T cells induced by MLPC has not been studied extensively yet. Therefore, we aimed to explore the function of tetramer+ CD8+ T cells generated by MLPC. We confirmed that population of tetramer positive cells in MLPC produced IFN-γ by recall stimulation, using intracellular cytokine staining. This study showed that WT1 positive cells, which were induced by MLPC, have function of producing IFN-γ and killing target cells. The present findings suggest that WT1 peptide immunized persons hold WT1 specific CTLs and IFN-γ production and cytotoxicity in CTLs could be enhanced by further WT1 peptide vaccination.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "164533", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Oiwa, Eri"}]}, {"nameIdentifiers": [{"nameIdentifier": "164534", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Narita, Miwako"}]}, {"nameIdentifiers": [{"nameIdentifier": "164535", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwaya, Shunpei"}]}, {"nameIdentifiers": [{"nameIdentifier": "164536", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nishizawa, Yoshinori"}]}, {"nameIdentifiers": [{"nameIdentifier": "164537", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Uchiyama, Takayoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "164538", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwabuchi, Minami"}]}, {"nameIdentifiers": [{"nameIdentifier": "164539", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Takahashi, Masuhiro"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学医学部保健学科"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12680484", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "21884617", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "大岩, 恵理"}], "nameIdentifiers": [{"nameIdentifier": "164526", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "成田, 美和子"}], "nameIdentifiers": [{"nameIdentifier": "164527", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩谷, 俊平"}], "nameIdentifiers": [{"nameIdentifier": "164528", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "西澤, 幹則"}], "nameIdentifiers": [{"nameIdentifier": "164529", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "内山, 孝由"}], "nameIdentifiers": [{"nameIdentifier": "164530", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩渕, 南"}], "nameIdentifiers": [{"nameIdentifier": "164531", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋, 益廣"}], "nameIdentifiers": [{"nameIdentifier": "164532", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "11(1)_57-65.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "11(1)_57-65.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28578/files/11(1)_57-65.pdf"}, "version_id": "92c09584-5e1a-4957-8a27-31bb8bb8c00d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "WT1抗原", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞傷害性T細胞 （CTL）", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ペプチドワクチン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IFN-γ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞傷害活性", "subitem_subject_scheme": "Other"}, {"subitem_subject": "WT1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytotoxic T lymphocyte （CTL）", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peptide vaccine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytotoxicity", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定"}, {"subitem_title": "WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1630"], "permalink_uri": "http://hdl.handle.net/10191/38934", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-02-29"}, "publish_date": "2016-02-29", "publish_status": "0", "recid": "28578", "relation": {}, "relation_version_is_last": true, "title": ["WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定"], "weko_shared_id": null}