{"created":"2021-03-01T06:34:24.305940+00:00","id":28578,"links":{},"metadata":{"_buckets":{"deposit":"92f1c469-5fe3-4375-94b4-4feb072ba774"},"_deposit":{"id":"28578","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28578"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028578","sets":["453:456","506:1624:1625:1630"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Identification of IFN-γ production and cytotoxicity in WT1 tetramer+ T cells generated by mixed lymphocyte peptide culture in WT1 peptide immunized persons"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"65","bibliographicPageStart":"57","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"新潟大学保健学雑誌"},{"bibliographic_title":"新潟大学保健学雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"抗腫瘍免疫療法施行例における治療効果を判定するために，in vitroで培養された腫瘍抗原特異的細胞傷害性T細胞（CTL）が特異的に抗原を認識し，細胞傷害機能を有するか調べることを目的とした。本検討では，in vivoに存在するWT1特異的CTLを混合リンパ球ペプチド培養（mixed lymphocyte peptide culture; MLPC）によりin vitroで増幅し，WT1特異的CTLの機能解析を行った。in vitroで誘導されたWT1特異的CTLはWT1抗原特異的にIFN-γを合成し，細胞傷害活性を有することが確認された。以上のことから，WT1ペプチドワクチン施行例の末梢血中にはIFN-γの産生能と細胞傷害活性を持つWT1特異的CTLが存在し，これらのCTLはさらなるWT1ペプチドワクチンにより増幅されると考えられた。","subitem_description_type":"Abstract"},{"subitem_description":"Although the frequencies of cytotoxic T lymphocytes（CTLs）in peripheral blood（PB）can be evaluated by mixed lymphocyte peptide culture（MLPC）, the function of tetramer+ CD8+ T cells induced by MLPC has not been studied extensively yet. Therefore, we aimed to explore the function of tetramer+ CD8+ T cells generated by MLPC. We confirmed that population of tetramer positive cells in MLPC produced IFN-γ by recall stimulation, using intracellular cytokine staining. This study showed that WT1 positive cells, which were induced by MLPC, have function of producing IFN-γ and killing target cells. The present findings suggest that WT1 peptide immunized persons hold WT1 specific CTLs and IFN-γ production and cytotoxicity in CTLs could be enhanced by further WT1 peptide vaccination.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"164533","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Oiwa, Eri"}]},{"nameIdentifiers":[{"nameIdentifier":"164534","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Miwako"}]},{"nameIdentifiers":[{"nameIdentifier":"164535","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwaya, Shunpei"}]},{"nameIdentifiers":[{"nameIdentifier":"164536","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nishizawa, Yoshinori"}]},{"nameIdentifiers":[{"nameIdentifier":"164537","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Uchiyama, Takayoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"164538","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwabuchi, Minami"}]},{"nameIdentifiers":[{"nameIdentifier":"164539","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masuhiro"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学医学部保健学科"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12680484","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21884617","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大岩, 恵理"}],"nameIdentifiers":[{"nameIdentifier":"164526","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"成田, 美和子"}],"nameIdentifiers":[{"nameIdentifier":"164527","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩谷, 俊平"}],"nameIdentifiers":[{"nameIdentifier":"164528","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西澤, 幹則"}],"nameIdentifiers":[{"nameIdentifier":"164529","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"内山, 孝由"}],"nameIdentifiers":[{"nameIdentifier":"164530","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩渕, 南"}],"nameIdentifiers":[{"nameIdentifier":"164531","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 益廣"}],"nameIdentifiers":[{"nameIdentifier":"164532","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"11(1)_57-65.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"11(1)_57-65.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28578/files/11(1)_57-65.pdf"},"version_id":"92c09584-5e1a-4957-8a27-31bb8bb8c00d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"WT1抗原","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害性T細胞 （CTL）","subitem_subject_scheme":"Other"},{"subitem_subject":"ペプチドワクチン","subitem_subject_scheme":"Other"},{"subitem_subject":"IFN-γ","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害活性","subitem_subject_scheme":"Other"},{"subitem_subject":"WT1","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxic T lymphocyte （CTL）","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide vaccine","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxicity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定"},{"subitem_title":"WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1630"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-29"},"publish_date":"2016-02-29","publish_status":"0","recid":"28578","relation_version_is_last":true,"title":["WT1ペプチドワクチン施行症例におけるWT1特異的細胞傷害性T細胞の検出及びそのIFN-γ産生能と細胞傷害活性の同定"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:46.008717+00:00"}