{"_buckets": {"deposit": "62b99921-c30d-46fc-bf35-d9e21d5b3351"}, "_deposit": {"id": "2787", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2787"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002787"}, "item_5_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Evaluation of Virus-free Bulblet Production by Antiviral and/or Heat Treatment in In Vitro Scale Cultures of Lilium longiflorum \u0027Georgia\u0027 and L. X \u0027Casablanca\u0027"}]}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "647", "bibliographicPageStart": "640", "bibliographicVolumeNumber": "68", "bibliographic_titles": [{"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "This study evaluated the effects of chemotherapy (addition of ribavirin or 2-thiouracil to a medium) and/or heat treatment at 35\u2103 on the production of virus-free bulblets in the scales culture of Lilium longiflorum \u0027Georgia\u0027 and L. x \u0027Casablanca\u0027, infected with lily symptomless virus (LSV), tulip breaking virus-lily (TBV-L), and cucumber mosaic virus (CMV).\\r\\\\n 1. When scales were cultured on a medium with ribavirin at 50\u03bcM, ELISA-absorbance values at 405nm for LSV in the incubated scales decreased as the incubation time lengthened, whereas the TBV-L values of the scales decreased at almost the same rate as those of the control.\\r\\\\n 2. When scales of L. longiflorum \u0027Georgia\u0027 were cultured on medium with antiviral chemicals, the number of bulblets formed decreased as concentrations of antiviral chemicals increased. Viruses were detected in about 20% of the bulblets at the end of culture when the scales were cultured on medium with ribavirin and 2-thiouracil at 50\u03bcM; however, more than 44% of the bulblets, which were transplanted into soil and cultivated in the greenhouse for 6 months, showed the positive reaction in viruses.\\r\\\\n 3. Scales excised from bulblets heat-treated at 35\u2103 for 4 weeks formed fewer bulblets than those of control, especially the scales of \u0027Georgia\u0027.\\r\\\\n 4. Chemotherapy in combination with thermotherapy was more effective in decreasing the number of virus-infected bulblets than was the single treatment. When scales, kept at 35\u2103 for 4 weeks, were cultured on medium with 5\u03bcM ribavirin, viruses were detected in 30% of the bulblets of \u0027Georgia\u0027 and 6% of those in \u0027Casablanca\u0027 at the end of in vitro culture. However, viruses were detected in 100% of the bulblets in \u0027Georgia\u0027 and 44% of those in \u0027Casablanca\u0027 which were transplanted into soil and cultivated in the greenhouse for 6 months.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u2018\u30b8\u30e7\u30fc\u30b8\u30a2\u2019\u3068\u2018\u30ab\u30b5\u30d6\u30e9\u30f3\u30ab\u2019\u306e\u308a\u3093\u7247\u57f9\u990a\u306b\u3088\u308b\u30a6\u30a4\u30eb\u30b9\u30d5\u30ea\u30fc\u7403\u306e\u52b9\u7387\u7684\u306a\u7372\u5f97\u306e\u305f\u3081\uff0c\u6297\u30a6\u30a4\u30eb\u30b9\u5264(ribavirin\u304a\u3088\u30732-thiouracil)\u51e6\u7406\uff0c \u71b1\u51e6\u7406\u3068\u4e21\u8005\u306e\u4f75\u7528\u51e6\u7406\u306e\u52b9\u679c\u3092\u691c\u8a0e\u3057\u305f\uff0e\\r\\\\n1\uff0e50\u03bcM 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