{"created":"2021-03-01T06:06:31.019929+00:00","id":2787,"links":{},"metadata":{"_buckets":{"deposit":"62b99921-c30d-46fc-bf35-d9e21d5b3351"},"_deposit":{"id":"2787","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2787"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002787"},"item_5_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"Evaluation of Virus-free Bulblet Production by Antiviral and/or Heat Treatment in In Vitro Scale Cultures of Lilium longiflorum 'Georgia' and L. 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When scales were cultured on a medium with ribavirin at 50\u03bcM, ELISA-absorbance values at 405nm for LSV in the incubated scales decreased as the incubation time lengthened, whereas the TBV-L values of the scales decreased at almost the same rate as those of the control.\\r\\\\n 2. When scales of L. longiflorum 'Georgia' were cultured on medium with antiviral chemicals, the number of bulblets formed decreased as concentrations of antiviral chemicals increased. Viruses were detected in about 20% of the bulblets at the end of culture when the scales were cultured on medium with ribavirin and 2-thiouracil at 50\u03bcM; however, more than 44% of the bulblets, which were transplanted into soil and cultivated in the greenhouse for 6 months, showed the positive reaction in viruses.\\r\\\\n 3. Scales excised from bulblets heat-treated at 35\u2103 for 4 weeks formed fewer bulblets than those of control, especially the scales of 'Georgia'.\\r\\\\n 4. Chemotherapy in combination with thermotherapy was more effective in decreasing the number of virus-infected bulblets than was the single treatment. When scales, kept at 35\u2103 for 4 weeks, were cultured on medium with 5\u03bcM ribavirin, viruses were detected in 30% of the bulblets of 'Georgia' and 6% of those in 'Casablanca' at the end of in vitro culture. However, viruses were detected in 100% of the bulblets in 'Georgia' and 44% of those in 'Casablanca' which were transplanted into soil and cultivated in the greenhouse for 6 months.","subitem_description_type":"Abstract"},{"subitem_description":"\u2018\u30b8\u30e7\u30fc\u30b8\u30a2\u2019\u3068\u2018\u30ab\u30b5\u30d6\u30e9\u30f3\u30ab\u2019\u306e\u308a\u3093\u7247\u57f9\u990a\u306b\u3088\u308b\u30a6\u30a4\u30eb\u30b9\u30d5\u30ea\u30fc\u7403\u306e\u52b9\u7387\u7684\u306a\u7372\u5f97\u306e\u305f\u3081\uff0c\u6297\u30a6\u30a4\u30eb\u30b9\u5264(ribavirin\u304a\u3088\u30732-thiouracil)\u51e6\u7406\uff0c \u71b1\u51e6\u7406\u3068\u4e21\u8005\u306e\u4f75\u7528\u51e6\u7406\u306e\u52b9\u679c\u3092\u691c\u8a0e\u3057\u305f\uff0e\\r\\\\n1\uff0e50\u03bcM 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