WEKO3
アイテム
Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY
http://hdl.handle.net/10191/1164
http://hdl.handle.net/10191/11644825fd8d-2ea4-4d0e-b3bf-3a085a3fcfaa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
24_0023.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-23 | |||||
| タイトル | ||||||
| タイトル | Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY | |||||
| タイトル | ||||||
| タイトル | Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cell line | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cell kinetics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | RNA quantitation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 7AAD | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PY | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Flow cytometry | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Toba, Ken
× Toba, Ken× Kishi, Kenji× Koike, Tadashi× Winton, Elliott F.× Takahashi, Hoyu× Nagai, Koichi× Maruyama, Soichi× Furukawa, Tatsuo× Hashimoto, Shigeo× Masuko, Masayoshi× Uesugi, Yumiko× Kuroha, Takashi× Tsukada, Nobuhiro× Shibata, Akira |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4848 | |||||
| 姓名 | 鳥羽, 健 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We analyzed the surface phenotypes and cell cycle of twenty two hemopoietic cell lines using 7AAD/PY based on the cellular DNA/RNA content. Populations of G1a, G1b, S, and G2+M, DNA index (DI), and the RNA index of S phase (SRI) were calculated by means of DNA/RNA dot plots. Two new parameters were extracted from the cell cycle profiles, namely, the nucleic acid index of the S phase (NI) and the coefficient of variations in the RNA at S phase (SCV). DNA/RNA dot plots of cell lines revealed four characteristic profiles of the cell cycle defined with the calculated NI and SCV. These were Type 0 (small NI, large SCV), Type I (small NI, small SCV), Type II (large NI, small SCV) and Type III (large NI, large SCV). Type 0 included four stem cell lines: a t(l;19) leukemia, two Ph1 positive ALL and a biphenotypic crisis of CGL. Type I included five ALL cell lines: three T-ALL and two common B-ALL. Type II contained ten myeloid cell lines: five AML and five myeloid crisis of CGL and Type III consisted of three relatively immature lymphoma cell lines: two Burkitt's lymphoma and a follicular center lymphoma cell line. Calculated (NI / SCV(%)) were as follows: Type 0 2.27±0.19 / 16.7±3.7; Type I 2.20±0.30 / 11.1±0.7; Type II 3.64±0.52 / 11.8±1.0 and Type III 3.60±0.53 / 17.5±1.9. Cell cycle analysis of blasts using 7AAD/PY combined with surface phenotyping may yield important and rapid information for the classification of hematopoietic malignancy within 2 hours of patient admission. | |||||
| 書誌情報 |
Experimental Hematology en : Experimental Hematology 巻 24, 号 8, p. 894-901, 発行日 1996-08 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0301472X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00641088 | |||||
| 権利 | ||||||
| 権利情報 | International Society for Experimental Hematology | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
