@article{oai:niigata-u.repo.nii.ac.jp:00001656,
 author = {Toba, Ken and Kishi, Kenji and Koike, Tadashi and Winton, Elliott F. and Takahashi, Hoyu and Nagai, Koichi and Maruyama, Soichi and Furukawa, Tatsuo and Hashimoto, Shigeo and Masuko, Masayoshi and Uesugi, Yumiko and Kuroha, Takashi and Tsukada, Nobuhiro and Shibata, Akira},
 issue = {8},
 journal = {Experimental Hematology, Experimental Hematology},
 month = {Aug},
 note = {We analyzed the surface phenotypes and cell cycle of twenty two hemopoietic cell lines using 7AAD/PY based on the cellular DNA/RNA content. Populations of G1a, G1b, S, and G2+M, DNA index (DI), and the RNA index of S phase (SRI) were calculated by means of DNA/RNA dot plots. Two new parameters were extracted from the cell cycle profiles, namely, the nucleic acid index of the S phase (NI) and the coefficient of variations in the RNA at S phase (SCV). DNA/RNA dot plots of cell lines revealed four characteristic profiles of the cell cycle defined with the calculated NI and SCV. These were Type 0 (small NI, large SCV), Type I (small NI, small SCV), Type II (large NI, small SCV) and Type III (large NI, large SCV). Type 0 included four stem cell lines: a t(l;19) leukemia, two Ph1 positive ALL and a biphenotypic crisis of CGL. Type I included five ALL cell lines: three T-ALL and two common B-ALL. Type II contained ten myeloid cell lines: five AML and five myeloid crisis of CGL and Type III consisted of three relatively immature lymphoma cell lines: two Burkitt's lymphoma and a follicular center lymphoma cell line. Calculated (NI / SCV(%)) were as follows: Type 0 2.27±0.19 / 16.7±3.7; Type I 2.20±0.30 / 11.1±0.7; Type II 3.64±0.52 / 11.8±1.0 and Type III 3.60±0.53 / 17.5±1.9. Cell cycle analysis of blasts using 7AAD/PY combined with surface phenotyping may yield important and rapid information for the classification of hematopoietic malignancy within 2 hours of patient admission.},
 pages = {894--901},
 title = {Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY},
 volume = {24},
 year = {1996}
}