{"created":"2021-03-01T06:05:19.723093+00:00","id":1656,"links":{},"metadata":{"_buckets":{"deposit":"6612c111-6179-4154-a9f7-c292ead7b1e2"},"_deposit":{"id":"1656","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1656"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001656","sets":["453:454","482:483:484"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"901","bibliographicPageStart":"894","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Experimental Hematology"},{"bibliographic_title":"Experimental Hematology","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We analyzed the surface phenotypes and cell cycle of twenty two hemopoietic cell lines using 7AAD/PY based on the cellular DNA/RNA content. Populations of G1a, G1b, S, and G2+M, DNA index (DI), and the RNA index of S phase (SRI) were calculated by means of DNA/RNA dot plots. Two new parameters were extracted from the cell cycle profiles, namely, the nucleic acid index of the S phase (NI) and the coefficient of variations in the RNA at S phase (SCV). DNA/RNA dot plots of cell lines revealed four characteristic profiles of the cell cycle defined with the calculated NI and SCV. These were Type 0 (small NI, large SCV), Type I (small NI, small SCV), Type II (large NI, small SCV) and Type III (large NI, large SCV). Type 0 included four stem cell lines: a t(l;19) leukemia, two Ph1 positive ALL and a biphenotypic crisis of CGL. Type I included five ALL cell lines: three T-ALL and two common B-ALL. Type II contained ten myeloid cell lines: five AML and five myeloid crisis of CGL and Type III consisted of three relatively immature lymphoma cell lines: two Burkitt's lymphoma and a follicular center lymphoma cell line. Calculated (NI / SCV(%)) were as follows: Type 0 2.27±0.19 / 16.7±3.7; Type I 2.20±0.30 / 11.1±0.7; Type II 3.64±0.52 / 11.8±1.0 and Type III 3.60±0.53 / 17.5±1.9. Cell cycle analysis of blasts using 7AAD/PY combined with surface phenotyping may yield important and rapid information for the classification of hematopoietic malignancy within 2 hours of patient admission.","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4848","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鳥羽, 健"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"International Society for Experimental Hematology"}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00641088","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301472X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Toba, Ken"}],"nameIdentifiers":[{"nameIdentifier":"4834","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kishi, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"4835","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"4836","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Winton, Elliott F."}],"nameIdentifiers":[{"nameIdentifier":"4837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Hoyu"}],"nameIdentifiers":[{"nameIdentifier":"4838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagai, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"4839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maruyama, Soichi"}],"nameIdentifiers":[{"nameIdentifier":"4840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Tatsuo"}],"nameIdentifiers":[{"nameIdentifier":"4841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Shigeo"}],"nameIdentifiers":[{"nameIdentifier":"4842","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masuko, Masayoshi"}],"nameIdentifiers":[{"nameIdentifier":"4843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uesugi, Yumiko"}],"nameIdentifiers":[{"nameIdentifier":"4844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuroha, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"4845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukada, Nobuhiro"}],"nameIdentifiers":[{"nameIdentifier":"4846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibata, Akira"}],"nameIdentifiers":[{"nameIdentifier":"4847","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"24_0023.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"24_0023.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1656/files/24_0023.pdf"},"version_id":"55eab820-822a-4e21-81be-7dbe4df2d149"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cell line","subitem_subject_scheme":"Other"},{"subitem_subject":"cell kinetics","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA quantitation","subitem_subject_scheme":"Other"},{"subitem_subject":"7AAD","subitem_subject_scheme":"Other"},{"subitem_subject":"PY","subitem_subject_scheme":"Other"},{"subitem_subject":"Flow cytometry","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY"},{"subitem_title":"Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","484"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"1656","relation_version_is_last":true,"title":["Profile of cell cycle in hemopoietic malignancy by DNA/RNA quantitation using 7AAD/PY"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:34:19.520751+00:00"}