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Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins
http://hdl.handle.net/10191/6327
http://hdl.handle.net/10191/63273504ed00-c7f9-4b7c-960c-5fdb42169a4d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_0003.pdf (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-23 | |||||
| タイトル | ||||||
| タイトル | Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Oryza sativa L. | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | membrane protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 2D-PAGE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NEPHGE | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Mikami, Satoshi
× Mikami, Satoshi× Kishimoto, Tadashi× Hori, Hidetaka× Mitsui, Toshiaki |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4327 | |||||
| 姓名 | 三ツ井, 敏明 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 66, 号 5, p. 1170-1173, 発行日 2002 |
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| 出版者 | ||||||
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8451 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824164 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1271/bbb.66.1170 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Mikami, Satoshi, Kishimoto, Tadashi, Hori, Hidetaka, Mitsui, Toshiaki, 2002, Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins: Japan Society for Bioscience, Biotechnology, and Agrochemistry, 1170–1173 p.
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