{"_buckets": {"deposit": "7fb323c2-4804-4a68-9714-573c6bd00f2c"}, "_deposit": {"id": "1564", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1564"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001564"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "1173", "bibliographicPageStart": "1170", "bibliographicVolumeNumber": "66", "bibliographic_titles": [{"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry"}, {"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "4327", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u4e09\u30c4\u4e95, \u654f\u660e"}]}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10824164", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09168451", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mikami, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "4323", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishimoto, Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "4324", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "4325", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "4326", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "07_0003.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "07_0003.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1564/files/07_0003.pdf"}, "version_id": "a07c0b8b-8bd7-478c-b4af-eead77eab5b7"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Oryza sativa L.", "subitem_subject_scheme": "Other"}, {"subitem_subject": "membrane protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "2D-PAGE", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NEPHGE", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins"}, {"subitem_title": "Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "485/486/487"], "permalink_uri": "http://hdl.handle.net/10191/6327", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-04-23"}, "publish_date": "2008-04-23", "publish_status": "0", "recid": "1564", "relation": {}, "relation_version_is_last": true, "title": ["Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins"], "weko_shared_id": null}