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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins

http://hdl.handle.net/10191/6327
http://hdl.handle.net/10191/6327
3504ed00-c7f9-4b7c-960c-5fdb42169a4d
名前 / ファイル ライセンス アクション
07_0003.pdf 07_0003.pdf (1.0 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
言語 en
タイトル Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins
言語
言語 eng
キーワード
主題Scheme Other
主題 Oryza sativa L.
キーワード
主題Scheme Other
主題 membrane protein
キーワード
主題Scheme Other
主題 2D-PAGE
キーワード
主題Scheme Other
主題 NEPHGE
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Mikami, Satoshi

× Mikami, Satoshi

WEKO 4323

en Mikami, Satoshi

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Kishimoto, Tadashi

× Kishimoto, Tadashi

WEKO 4324

en Kishimoto, Tadashi

Search repository
Hori, Hidetaka

× Hori, Hidetaka

WEKO 4325

en Hori, Hidetaka

Search repository
Mitsui, Toshiaki

× Mitsui, Toshiaki

WEKO 4326

en Mitsui, Toshiaki

Search repository
著者別名
識別子 4327
識別子Scheme WEKO
姓名 三ツ井, 敏明
言語 ja
抄録
内容記述タイプ Abstract
内容記述 Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.
言語 en
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 66, 号 5, p. 1170-1173, 発行日 2002
出版者
言語 en
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.66.1170
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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