{"created":"2021-03-01T06:05:14.120881+00:00","id":1564,"links":{},"metadata":{"_buckets":{"deposit":"dc3694ab-1fff-4d2a-8795-55ee8aff67ef"},"_deposit":{"id":"1564","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1564"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001564","sets":["453:454","485:486:487"]},"author_link":["4323","4324","4325","4326","4327"],"control_number":"1564","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1173","bibliographicPageStart":"1170","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4327","nameIdentifierScheme":"WEKO"}],"names":[{"name":"三ツ井, 敏明","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry","subitem_publisher_language":"en"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1271/bbb.66.1170","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mikami, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kishimoto, Tadashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4324","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Hidetaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4325","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsui, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4326","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"07_0003.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"07_0003.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1564/files/07_0003.pdf"},"version_id":"02a07cac-146e-479c-a493-54fe2c1b2582"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Oryza sativa L.","subitem_subject_scheme":"Other"},{"subitem_subject":"membrane protein","subitem_subject_scheme":"Other"},{"subitem_subject":"2D-PAGE","subitem_subject_scheme":"Other"},{"subitem_subject":"NEPHGE","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-23"},"publish_date":"2008-04-23","publish_status":"0","recid":"1564","relation_version_is_last":true,"title":["Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:23:27.771213+00:00"}