@article{oai:niigata-u.repo.nii.ac.jp:00001564,
 author = {Mikami, Satoshi and Kishimoto, Tadashi and Hori, Hidetaka and Mitsui, Toshiaki},
 issue = {5},
 journal = {Bioscience, Biotechnology, and Biochemistry},
 month = {},
 note = {Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.},
 pages = {1170--1173},
 title = {Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins},
 volume = {66},
 year = {2002}
}