{"_buckets": {"deposit": "e73598ca-f76a-4115-b392-f546058fc6eb"}, "_deposit": {"id": "10500", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10500"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00010500", "sets": ["456", "971"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "A Novel Mechanism for inhibition of Bone Morphogenetic Protein-2/4 Signaling by High Temperature Requirement Protein A1"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "28", "bibliographicPageStart": "16", "bibliographicVolumeNumber": "124", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "High temperature requirement protein A1 (HtrA1) はセリンプロテアーゼ活性を持つHtrAファミリータンパクの一員である. HtrA1は bone morphogenetic protein (BMP)-2及びBMP-4シグナル伝達を抑制するが, その機序は不明である. 筆者は, HtrA1がBMP-2/4前駆体を切断し, そのシグナル伝達を阻害するという仮説をたて, これを検証した. 培養細胞系を用い, HtrA1存在下でのBMP-2/4の量を検討した. さらに, 細胞混合アッセイを用い, その作用する場を検討した. また, 細胞内において, HtrA1由来のBMP-2/4前駆体の断片を検出した. 結果として, HtrA1存在下では, 培養液中の成熟型BMP-2/4の量が減少し, この効果はHtrA1とBMP-2/4前駆体が同一の細胞内に存在するときにのみ認められた. さらに, 小胞体において, HtrA1がBMP-2/4前駆体のC末端を切断することを示した. 成熟型BMP-2/4はBMP-2/4前駆体がゴルジ装置以降でfurinによるプロセッシングを受けて産生されるが, 本論文は, HtrA1がfurinによるプロセッシングに先行して小胞体でBMP-2/4前駆体を切断し, 成熟型BMP-2/4の量を減少させることで, そのシグナル伝達を阻害することを示した.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "71945", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nozaki, Hiroaki"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "野崎, 洋明"}], "nameIdentifiers": [{"nameIdentifier": "71944", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "124(1)_16-28.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "124(1)_16-28.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/10500/files/124(1)_16-28.pdf"}, "version_id": "a71baa04-25cd-471a-bdec-e3c0c2344fdc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "high temperature requirement protein A1 (HtrA1)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "bone morphogenetic proten (BMP)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "furin,endoplasmic reticulum (ER)", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序"}, {"subitem_title": "High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "971"], "permalink_uri": "http://hdl.handle.net/10191/28653", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-07-24"}, "publish_date": "2014-07-24", "publish_status": "0", "recid": "10500", "relation": {}, "relation_version_is_last": true, "title": ["High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序"], "weko_shared_id": null}