{"created":"2021-03-01T06:14:38.571796+00:00","id":10500,"links":{},"metadata":{"_buckets":{"deposit":"e73598ca-f76a-4115-b392-f546058fc6eb"},"_deposit":{"id":"10500","owners":[],"pid":{"revision_id":0,"type":"depid","value":"10500"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00010500","sets":["453:456","471:537:538:971"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A Novel Mechanism for inhibition of Bone Morphogenetic Protein-2/4 Signaling by High Temperature Requirement Protein A1"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"28","bibliographicPageStart":"16","bibliographicVolumeNumber":"124","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"High temperature requirement protein A1 (HtrA1) はセリンプロテアーゼ活性を持つHtrAファミリータンパクの一員である. HtrA1は bone morphogenetic protein (BMP)-2及びBMP-4シグナル伝達を抑制するが, その機序は不明である. 筆者は, HtrA1がBMP-2/4前駆体を切断し, そのシグナル伝達を阻害するという仮説をたて, これを検証した. 培養細胞系を用い, HtrA1存在下でのBMP-2/4の量を検討した. さらに, 細胞混合アッセイを用い, その作用する場を検討した. また, 細胞内において, HtrA1由来のBMP-2/4前駆体の断片を検出した. 結果として, HtrA1存在下では, 培養液中の成熟型BMP-2/4の量が減少し, この効果はHtrA1とBMP-2/4前駆体が同一の細胞内に存在するときにのみ認められた. さらに, 小胞体において, HtrA1がBMP-2/4前駆体のC末端を切断することを示した. 成熟型BMP-2/4はBMP-2/4前駆体がゴルジ装置以降でfurinによるプロセッシングを受けて産生されるが, 本論文は, HtrA1がfurinによるプロセッシングに先行して小胞体でBMP-2/4前駆体を切断し, 成熟型BMP-2/4の量を減少させることで, そのシグナル伝達を阻害することを示した.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"71945","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nozaki, Hiroaki"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野崎, 洋明"}],"nameIdentifiers":[{"nameIdentifier":"71944","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"124(1)_16-28.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"124(1)_16-28.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/10500/files/124(1)_16-28.pdf"},"version_id":"a71baa04-25cd-471a-bdec-e3c0c2344fdc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"high temperature requirement protein A1 (HtrA1)","subitem_subject_scheme":"Other"},{"subitem_subject":"bone morphogenetic proten (BMP)","subitem_subject_scheme":"Other"},{"subitem_subject":"furin,endoplasmic reticulum (ER)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序"},{"subitem_title":"High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","971"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-24"},"publish_date":"2014-07-24","publish_status":"0","recid":"10500","relation_version_is_last":true,"title":["High Temperature Requirement Protein A1による Bone Morphogenetic Protein-2/4シグナル伝達の阻害機序"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:44:11.166650+00:00"}