{"_buckets": {"deposit": "cd0ed172-cf84-4028-b56f-15bdfe7244c3"}, "_deposit": {"id": "10477", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10477"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00010477", "sets": ["456", "970"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Rat IL-1 Receptor-Ig Heterodimer as a IL-1 Inhibitor"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "75", "bibliographicPageStart": "65", "bibliographicVolumeNumber": "124", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "生物学的製剤は, 自己免疫疾患の治療に大きなパラダイムシフトを起こしつつあり, 他の疾患にもその有用性がいくつか報告されてきている. その中でも, IL-1は様々な炎症の中心的役割を担うサイトカインの一つであり, IL-1を阻害する生物学的製剤は, 幅広い疾患に有効性を示す可能性がある. 今回, 私は, IL-1受容体タイプII (IL-1RII) -IgFcとIL-1受容体アクセサリー蛋白 (IL-1Racp)-IgFcのヘテロダイマーを作成し, そのIL-1阻害作用を検討した. pCAGGSプラスミドベクターにIL-1RII-IgFcとIL-1Racp-IgFcをそれぞれ挿入したものを作成し, Cos7培養細胞に遺伝子導入して得られた培養上清を用いてIL-1阻害作用を検討した. IL-1阻害作用は, NRK49F培養細胞にIL-1αとそれぞれのCos7培養上清を添加し, そのMCP-1の遺伝子発現の変化を定量的RT-PCRで測定し, 判定した. IL-1阻害作用は, それぞれのホモダイマーではあまりみられず, ヘテロダイマーだけに非常に強いIL-1阻害作用がみられた. その作用は, 現在海外で市販されはじめたアナキンラの約10000倍強力であり, 新しい生物学的製剤として期待できると考えられた.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "71828", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Otaki, Keita"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "大瀧, 啓太"}], "nameIdentifiers": [{"nameIdentifier": "71827", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "124(2)_65-75.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "124(2)_65-75.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/10477/files/124(2)_65-75.pdf"}, "version_id": "697620b7-db29-436c-83ff-2b7b5a42d3ac"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "生物学的製剤", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IL-1阻害薬", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IL-1受容体へテロダイマー", "subitem_subject_scheme": "Other"}, {"subitem_subject": "自己免疫疾患", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ラットIL-1受容体-IgへテロダイマーによるIL-1阻害作用の検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ラットIL-1受容体-IgへテロダイマーによるIL-1阻害作用の検討"}, {"subitem_title": "ラットIL-1受容体-IgへテロダイマーによるIL-1阻害作用の検討", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "970"], "permalink_uri": "http://hdl.handle.net/10191/28641", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-07-24"}, "publish_date": "2014-07-24", "publish_status": "0", "recid": "10477", "relation": {}, "relation_version_is_last": true, "title": ["ラットIL-1受容体-IgへテロダイマーによるIL-1阻害作用の検討"], "weko_shared_id": null}