WEKO3
アイテム
A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B
http://hdl.handle.net/10191/33541
http://hdl.handle.net/10191/3354123ee22df-686f-417e-8a74-c3a9df7fe21c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
37(2)_79-84.pdf (748.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-10-06 | |||||
| タイトル | ||||||
| タイトル | A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B | |||||
| タイトル | ||||||
| タイトル | A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Shinada, Shoji
× Shinada, Shoji |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A procedure to prepare substrate platelets for fluorocytometry was developed using a plastic pipet column packed with Sepharose 2B and plastic microtubes. The gel fractionation proved to be reliable in collecting platelets free from plasma. Collected platelets were centrifuged with ACD, incubated with ammonium oxalate, centrifuged and resuspended in phosphate buffered saline as substrate platelets. Reactions between the substrate platelets obtained from blood group-0 healthy men and alloimmunized anti-platelet antibodies, i.e., anti-Yuk^a and anti-Yuk^b serum, were analyzed by a laser flow cytometer, Cytoron (Ortho Diagnostic Systems), with satisfying results. This preparation procedure seems to be useful for fluorocytometric analyses of platelets and has advantages of being able to reduce both overall assay time and blood volume. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 37, 号 2, p. 79-84, 発行日 1989-09 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
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| 値 | publisher | |||||
