{"_buckets": {"deposit": "ff7b996c-567a-4098-90c4-10970199a9be"}, "_deposit": {"id": "6647", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6647"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006647"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1989-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "84", "bibliographicPageStart": "79", "bibliographicVolumeNumber": "37", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "A procedure to prepare substrate platelets for fluorocytometry was developed using a plastic pipet column packed with Sepharose 2B and plastic microtubes. The gel fractionation proved to be reliable in collecting platelets free from plasma. Collected platelets were centrifuged with ACD, incubated with ammonium oxalate, centrifuged and resuspended in phosphate buffered saline as substrate platelets. Reactions between the substrate platelets obtained from blood group-0 healthy men and alloimmunized anti-platelet antibodies, i.e., anti-Yuk^a and anti-Yuk^b serum, were analyzed by a laser flow cytometer, Cytoron (Ortho Diagnostic Systems), with satisfying results. This preparation procedure seems to be useful for fluorocytometric analyses of platelets and has advantages of being able to reduce both overall assay time and blood volume.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shinada, Shoji"}], "nameIdentifiers": [{"nameIdentifier": "53805", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "37(2)_79-84.pdf", "filesize": [{"value": "748.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 748000.0, "url": {"label": "37(2)_79-84.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6647/files/37(2)_79-84.pdf"}, "version_id": "be708cc7-1890-41a8-8362-62e6cc0a4b1c"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B"}, {"subitem_title": "A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/649"], "permalink_uri": "http://hdl.handle.net/10191/33541", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-10-06"}, "publish_date": "2015-10-06", "publish_status": "0", "recid": "6647", "relation": {}, "relation_version_is_last": true, "title": ["A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B"], "weko_shared_id": null}