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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.37 No.2

A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B

http://hdl.handle.net/10191/33541
http://hdl.handle.net/10191/33541
23ee22df-686f-417e-8a74-c3a9df7fe21c
名前 / ファイル ライセンス アクション
37(2)_79-84.pdf 37(2)_79-84.pdf (748.0 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-10-06
タイトル
タイトル A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B
タイトル
タイトル A Procedure to Prepare Human Platelets for Fluorocytometric Analyses Using a Pipet Column Packed with Sepharose 2B
言語 en
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Shinada, Shoji

× Shinada, Shoji

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Shinada, Shoji

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内容記述タイプ Abstract
内容記述 A procedure to prepare substrate platelets for fluorocytometry was developed using a plastic pipet column packed with Sepharose 2B and plastic microtubes. The gel fractionation proved to be reliable in collecting platelets free from plasma. Collected platelets were centrifuged with ACD, incubated with ammonium oxalate, centrifuged and resuspended in phosphate buffered saline as substrate platelets. Reactions between the substrate platelets obtained from blood group-0 healthy men and alloimmunized anti-platelet antibodies, i.e., anti-Yuk^a and anti-Yuk^b serum, were analyzed by a laser flow cytometer, Cytoron (Ortho Diagnostic Systems), with satisfying results. This preparation procedure seems to be useful for fluorocytometric analyses of platelets and has advantages of being able to reduce both overall assay time and blood volume.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 37, 号 2, p. 79-84, 発行日 1989-09
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
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