{"_buckets": {"deposit": "ba5d4cb3-69ff-42ed-a413-0bd867f84f2f"}, "_deposit": {"id": "6594", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6594"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006594"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1991-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "127", "bibliographicPageStart": "119", "bibliographicVolumeNumber": "39", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Two human myelomonocytie leukemia cell lines highly resistant to 1-\u03b2-D-arabinofuranosyleytosine (ara-C) were established. One line, designated KY-Ra, has been proliferating with 1 \u00d7 10^\u003c-4\u003e M ara-C for over one year. The other one, KY-Rb, has proliferated with 1 \u00d7 10^\u003c-5\u003e M N^4-behenoyl-1-\u03b2-D-arabinofuranosylcytosine (BH-AC) after it reached a proliferative capacity with 1\u00d710^\u003c-6\u003e M ara-C. These two area-C-resistant cell lines have shown approximately 5,900 fold and 18,000 fold resistance to ara-C over the parental KY-821 cell line. The deoxycytidine kinase activity of each cell line was 0.39 (dCMP/min/\u03bcg protein) for KY-Ra, 0.26 for KY-Rb, 1.23 for KY-821. Deoxycytidine deaminase activities were almost identical. In drug accumulations, arac-C resistant cell lines showed a reduced uptake of ara-C during a 4 h exposure to 1 nM ara-C. After loading almost the same intracellular contents of drugs, ara-CTP accumulation decreased in two ara-C resistant cell lines, and the efflux rate was increased in KY-Ra. These results indicate that the decreased deoxycytidine kinase activity, which resulted in a decreased ara-CTP accumulation, is one of major mechanisms of the resistance, and suggest the possibility that an increase of the outward flux of ara-C is one mechanism of resistance. These cell lines may be useful for studying the refractoriness encountered in ara-C administration.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kakihara, Toshio"}], "nameIdentifiers": [{"nameIdentifier": "53613", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "39(3)_119-127.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "39(3)_119-127.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6594/files/39(3)_119-127.pdf"}, "version_id": "18d9aeda-e73d-4531-9c11-ff350e91bcaf"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-\u03b2-D-Arabinofuranosylcytosine", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-\u03b2-D-Arabinofuranosylcytosine"}, {"subitem_title": "The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-\u03b2-D-Arabinofuranosylcytosine", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/640"], "permalink_uri": "http://hdl.handle.net/10191/33445", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-09-28"}, "publish_date": "2015-09-28", "publish_status": "0", "recid": "6594", "relation": {}, "relation_version_is_last": true, "title": ["The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-\u03b2-D-Arabinofuranosylcytosine"], "weko_shared_id": null}