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The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine
http://hdl.handle.net/10191/33445
http://hdl.handle.net/10191/334455735199a-1ca1-4062-b99e-49818a32f726
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
39(3)_119-127.pdf (2.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-09-28 | |||||
| タイトル | ||||||
| タイトル | The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine | |||||
| タイトル | ||||||
| タイトル | The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Kakihara, Toshio
× Kakihara, Toshio |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Two human myelomonocytie leukemia cell lines highly resistant to 1-β-D-arabinofuranosyleytosine (ara-C) were established. One line, designated KY-Ra, has been proliferating with 1 × 10^<-4> M ara-C for over one year. The other one, KY-Rb, has proliferated with 1 × 10^<-5> M N^4-behenoyl-1-β-D-arabinofuranosylcytosine (BH-AC) after it reached a proliferative capacity with 1×10^<-6> M ara-C. These two area-C-resistant cell lines have shown approximately 5,900 fold and 18,000 fold resistance to ara-C over the parental KY-821 cell line. The deoxycytidine kinase activity of each cell line was 0.39 (dCMP/min/μg protein) for KY-Ra, 0.26 for KY-Rb, 1.23 for KY-821. Deoxycytidine deaminase activities were almost identical. In drug accumulations, arac-C resistant cell lines showed a reduced uptake of ara-C during a 4 h exposure to 1 nM ara-C. After loading almost the same intracellular contents of drugs, ara-CTP accumulation decreased in two ara-C resistant cell lines, and the efflux rate was increased in KY-Ra. These results indicate that the decreased deoxycytidine kinase activity, which resulted in a decreased ara-CTP accumulation, is one of major mechanisms of the resistance, and suggest the possibility that an increase of the outward flux of ara-C is one mechanism of resistance. These cell lines may be useful for studying the refractoriness encountered in ara-C administration. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 39, 号 3, p. 119-127, 発行日 1991-09 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
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| 値 | publisher | |||||
