{"created":"2021-03-01T06:10:30.499277+00:00","id":6594,"links":{},"metadata":{"_buckets":{"deposit":"ba5d4cb3-69ff-42ed-a413-0bd867f84f2f"},"_deposit":{"id":"6594","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6594"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006594","sets":["453:456","471:537:568:640"]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"127","bibliographicPageStart":"119","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Acta medica et biologica"},{"bibliographic_title":"Acta medica et biologica","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two human myelomonocytie leukemia cell lines highly resistant to 1-β-D-arabinofuranosyleytosine (ara-C) were established. One line, designated KY-Ra, has been proliferating with 1 × 10^<-4> M ara-C for over one year. The other one, KY-Rb, has proliferated with 1 × 10^<-5> M N^4-behenoyl-1-β-D-arabinofuranosylcytosine (BH-AC) after it reached a proliferative capacity with 1×10^<-6> M ara-C. These two area-C-resistant cell lines have shown approximately 5,900 fold and 18,000 fold resistance to ara-C over the parental KY-821 cell line. The deoxycytidine kinase activity of each cell line was 0.39 (dCMP/min/μg protein) for KY-Ra, 0.26 for KY-Rb, 1.23 for KY-821. Deoxycytidine deaminase activities were almost identical. In drug accumulations, arac-C resistant cell lines showed a reduced uptake of ara-C during a 4 h exposure to 1 nM ara-C. After loading almost the same intracellular contents of drugs, ara-CTP accumulation decreased in two ara-C resistant cell lines, and the efflux rate was increased in KY-Ra. These results indicate that the decreased deoxycytidine kinase activity, which resulted in a decreased ara-CTP accumulation, is one of major mechanisms of the resistance, and suggest the possibility that an increase of the outward flux of ara-C is one mechanism of resistance. These cell lines may be useful for studying the refractoriness encountered in ara-C administration.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Niigata University School of Medicine"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508361","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05677734","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kakihara, Toshio"}],"nameIdentifiers":[{"nameIdentifier":"53613","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"39(3)_119-127.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"39(3)_119-127.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/6594/files/39(3)_119-127.pdf"},"version_id":"18d9aeda-e73d-4531-9c11-ff350e91bcaf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine"},{"subitem_title":"The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","640"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-09-28"},"publish_date":"2015-09-28","publish_status":"0","recid":"6594","relation_version_is_last":true,"title":["The Characterization of Highly Resistant Human Leukemic Cell Lines to 1-β-D-Arabinofuranosylcytosine"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:39:55.489806+00:00"}