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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.43 No.1

HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets

http://hdl.handle.net/10191/33198
http://hdl.handle.net/10191/33198
88da4166-9640-4a60-a5c5-e6cb1926def5
名前 / ファイル ライセンス アクション
43(1)_59-62.pdf 43(1)_59-62.pdf (548.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-31
タイトル
タイトル HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets
タイトル
言語 en
タイトル HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Kimura, Satoshi

× Kimura, Satoshi

WEKO 53145

Kimura, Satoshi

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Sugita, Osamu

× Sugita, Osamu

WEKO 53146

Sugita, Osamu

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Matsuto, Takayuki

× Matsuto, Takayuki

WEKO 53147

Matsuto, Takayuki

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Okada, Masahiko

× Okada, Masahiko

WEKO 53148

Okada, Masahiko

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Igarashi, Yasuyuki

× Igarashi, Yasuyuki

WEKO 53149

Igarashi, Yasuyuki

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抄録
内容記述タイプ Abstract
内容記述 A new method for the complete separation of polyphosphoinositides with a single high performance liquid chromatography (HPLC) run was applied to human platelets and cell line A431. The amounts of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4, 5-bisphosphate (PIP_2) were traced with ^<32>P before and after stimulation. With stimulation by thrombin, increased uptakes of ^<32>P to PI, PIP, and PIP_2 were observed in platelets within 3 min. A similar effect was observed in A431 cells with stimulation by epidermal growth factor. Preincubation with platelet aggregation inhibitors such as hirudin and N, N, N-trimethylsphingosine (an artificial sphingosine derivative) did not produce an increase of PI, PIP, or PIP_2 under stimulation with thrombin. Since phosphoinositides play important roles in signal transduction inside cells, our method should be useful for automatic quantitative analysis and large-scale purification of phosphoinositides.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 43, 号 1, p. 59-62, 発行日 1995-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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