{"_buckets": {"deposit": "78c36d5d-857c-4197-8967-45e4fb9b3be2"}, "_deposit": {"id": "6484", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6484"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006484"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1995-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "62", "bibliographicPageStart": "59", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "A new method for the complete separation of polyphosphoinositides with a single high performance liquid chromatography (HPLC) run was applied to human platelets and cell line A431. The amounts of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4, 5-bisphosphate (PIP_2) were traced with ^\u003c32\u003eP before and after stimulation. With stimulation by thrombin, increased uptakes of ^\u003c32\u003eP to PI, PIP, and PIP_2 were observed in platelets within 3 min. A similar effect was observed in A431 cells with stimulation by epidermal growth factor. Preincubation with platelet aggregation inhibitors such as hirudin and N, N, N-trimethylsphingosine (an artificial sphingosine derivative) did not produce an increase of PI, PIP, or PIP_2 under stimulation with thrombin. Since phosphoinositides play important roles in signal transduction inside cells, our method should be useful for automatic quantitative analysis and large-scale purification of phosphoinositides.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kimura, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "53145", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugita, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "53146", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuto, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "53147", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okada, Masahiko"}], "nameIdentifiers": [{"nameIdentifier": "53148", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Igarashi, Yasuyuki"}], "nameIdentifiers": [{"nameIdentifier": "53149", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "43(1)_59-62.pdf", "filesize": [{"value": "548.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 548200.0, "url": {"label": "43(1)_59-62.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6484/files/43(1)_59-62.pdf"}, "version_id": "3e0145b5-485d-4a10-9054-6b243a10bf7c"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets"}, {"subitem_title": "HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/624"], "permalink_uri": "http://hdl.handle.net/10191/33198", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-08-31"}, "publish_date": "2015-08-31", "publish_status": "0", "recid": "6484", "relation": {}, "relation_version_is_last": true, "title": ["HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets"], "weko_shared_id": null}