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HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets
http://hdl.handle.net/10191/33198
http://hdl.handle.net/10191/3319888da4166-9640-4a60-a5c5-e6cb1926def5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
43(1)_59-62.pdf (548.2 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-08-31 | |||||
| タイトル | ||||||
| タイトル | HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets | |||||
| タイトル | ||||||
| タイトル | HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Kimura, Satoshi
× Kimura, Satoshi× Sugita, Osamu× Matsuto, Takayuki× Okada, Masahiko× Igarashi, Yasuyuki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A new method for the complete separation of polyphosphoinositides with a single high performance liquid chromatography (HPLC) run was applied to human platelets and cell line A431. The amounts of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4, 5-bisphosphate (PIP_2) were traced with ^<32>P before and after stimulation. With stimulation by thrombin, increased uptakes of ^<32>P to PI, PIP, and PIP_2 were observed in platelets within 3 min. A similar effect was observed in A431 cells with stimulation by epidermal growth factor. Preincubation with platelet aggregation inhibitors such as hirudin and N, N, N-trimethylsphingosine (an artificial sphingosine derivative) did not produce an increase of PI, PIP, or PIP_2 under stimulation with thrombin. Since phosphoinositides play important roles in signal transduction inside cells, our method should be useful for automatic quantitative analysis and large-scale purification of phosphoinositides. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 43, 号 1, p. 59-62, 発行日 1995-03 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
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| 値 | publisher | |||||
