{"created":"2021-03-01T06:10:23.591345+00:00","id":6484,"links":{},"metadata":{"_buckets":{"deposit":"78c36d5d-857c-4197-8967-45e4fb9b3be2"},"_deposit":{"id":"6484","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6484"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006484","sets":["453:456","471:537:568:624"]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"62","bibliographicPageStart":"59","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Acta medica et biologica"},{"bibliographic_title":"Acta medica et biologica","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A new method for the complete separation of polyphosphoinositides with a single high performance liquid chromatography (HPLC) run was applied to human platelets and cell line A431. The amounts of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4, 5-bisphosphate (PIP_2) were traced with ^<32>P before and after stimulation. With stimulation by thrombin, increased uptakes of ^<32>P to PI, PIP, and PIP_2 were observed in platelets within 3 min. A similar effect was observed in A431 cells with stimulation by epidermal growth factor. Preincubation with platelet aggregation inhibitors such as hirudin and N, N, N-trimethylsphingosine (an artificial sphingosine derivative) did not produce an increase of PI, PIP, or PIP_2 under stimulation with thrombin. Since phosphoinositides play important roles in signal transduction inside cells, our method should be useful for automatic quantitative analysis and large-scale purification of phosphoinositides.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Niigata University School of Medicine"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508361","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05677734","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kimura, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"53145","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugita, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"53146","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuto, Takayuki"}],"nameIdentifiers":[{"nameIdentifier":"53147","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"53148","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Igarashi, Yasuyuki"}],"nameIdentifiers":[{"nameIdentifier":"53149","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"43(1)_59-62.pdf","filesize":[{"value":"548.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"43(1)_59-62.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/6484/files/43(1)_59-62.pdf"},"version_id":"3e0145b5-485d-4a10-9054-6b243a10bf7c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets"},{"subitem_title":"HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","624"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-08-31"},"publish_date":"2015-08-31","publish_status":"0","recid":"6484","relation_version_is_last":true,"title":["HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:39:46.707548+00:00"}