{"_buckets": {"deposit": "1562913e-e715-451f-9deb-a05970c19669"}, "_deposit": {"id": "6209", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6209"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006209", "sets": ["456", "579"]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "75", "bibliographicPageStart": "67", "bibliographicVolumeNumber": "54", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Lymph node metastasis is a major prognostic factor for esophageal cancer patients. However, the molecular mechanisms underlying node metastasis remain unclear. Vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor-D (VEGF-D), which are ligands for VEGFR-3, have been reported to be capable of stimulating lymphangiogenesis under in vivo experimental conditions. The aim of the present study was to measure VEGF-C and/or VEGF-D mRNA expression in clinical specimens of esophageal squamous cell carcinoma, and to examine the correlation between VEGF-C or VEGF-D messenger ribonucleic acid (mRNA) expression and conventional clinicopathological parameters, especially the lymphatic involvement of esophageal squamous cell carcinoma. As a previous study has demonstrated that an expression pattern of high VEGF-C and low VEGF-D is correlated with both lymph node metastasis and lymphatic invasion of cancer cells, we also investigated the VEGF-C/D ratio. Total RNAs were isolated from 51 surgical specimens of esophageal carcinoma tissue and 42 normal esophageal mucosae. The relative mRNA abundance of VEGF-C and VEGF-D was measured by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis, and mRNA expression of VEGF-C and VEGF-D was standardized against glceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression. VEGF-C mRNA was expressed similarly in esophageal carcinoma tissue and normal mucosal tissue ; however, VEGF-D mRNA expression was significantly lower in carcinoma tissue compared with normal mucosal tissue, and therefore the VEGF-C/VEGF-D ratio was significantly increased in tumors compared with normal mucosae. However, neither mRNA expression of VEGF-C or VEGF-D nor the VEGF-C/VEGF-D ratio correlated with any clinicopathological factors, including lymphatic invasion, venous invasion, lymph node status, and tumor stage. VEGF-C and VEGF-D gene expression do not appear to be involved in the lymphatic progression of esophageal carcinoma.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "本文データは学協会の許諾に基づきCiNiiから複製したものである"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kaneko, Kouji"}], "nameIdentifiers": [{"nameIdentifier": "51666", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koyama, Yu"}], "nameIdentifiers": [{"nameIdentifier": "51667", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanda, Tatsuo"}], "nameIdentifiers": [{"nameIdentifier": "51668", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Honma, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "51669", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohashi, Manabu"}], "nameIdentifiers": [{"nameIdentifier": "51670", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakagawa, Satoru"}], "nameIdentifiers": [{"nameIdentifier": "51671", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hatakeyama, Katsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "51672", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "110006483824.pdf", "filesize": [{"value": "746.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 746800.0, "url": {"label": "110006483824.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6209/files/110006483824.pdf"}, "version_id": "41a2c19a-bca7-4ae8-81bc-871b66c42ce5"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "VEGF-C", "subitem_subject_scheme": "Other"}, {"subitem_subject": "VEGF-D", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mRNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "esophageal carcinoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RT-PCR", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma"}, {"subitem_title": "mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "579"], "permalink_uri": "http://hdl.handle.net/10191/19588", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-09-28"}, "publish_date": "2012-09-28", "publish_status": "0", "recid": "6209", "relation": {}, "relation_version_is_last": true, "title": ["mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma"], "weko_shared_id": null}