{"created":"2021-03-01T06:10:06.157891+00:00","id":6209,"links":{},"metadata":{"_buckets":{"deposit":"1562913e-e715-451f-9deb-a05970c19669"},"_deposit":{"id":"6209","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6209"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006209","sets":["453:456","471:537:568:579"]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"75","bibliographicPageStart":"67","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"Acta medica et biologica"},{"bibliographic_title":"Acta medica et biologica","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lymph node metastasis is a major prognostic factor for esophageal cancer patients. However, the molecular mechanisms underlying node metastasis remain unclear. Vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor-D (VEGF-D), which are ligands for VEGFR-3, have been reported to be capable of stimulating lymphangiogenesis under in vivo experimental conditions. The aim of the present study was to measure VEGF-C and/or VEGF-D mRNA expression in clinical specimens of esophageal squamous cell carcinoma, and to examine the correlation between VEGF-C or VEGF-D messenger ribonucleic acid (mRNA) expression and conventional clinicopathological parameters, especially the lymphatic involvement of esophageal squamous cell carcinoma. As a previous study has demonstrated that an expression pattern of high VEGF-C and low VEGF-D is correlated with both lymph node metastasis and lymphatic invasion of cancer cells, we also investigated the VEGF-C/D ratio. Total RNAs were isolated from 51 surgical specimens of esophageal carcinoma tissue and 42 normal esophageal mucosae. The relative mRNA abundance of VEGF-C and VEGF-D was measured by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis, and mRNA expression of VEGF-C and VEGF-D was standardized against glceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression. VEGF-C mRNA was expressed similarly in esophageal carcinoma tissue and normal mucosal tissue ; however, VEGF-D mRNA expression was significantly lower in carcinoma tissue compared with normal mucosal tissue, and therefore the VEGF-C/VEGF-D ratio was significantly increased in tumors compared with normal mucosae. However, neither mRNA expression of VEGF-C or VEGF-D nor the VEGF-C/VEGF-D ratio correlated with any clinicopathological factors, including lymphatic invasion, venous invasion, lymph node status, and tumor stage. VEGF-C and VEGF-D gene expression do not appear to be involved in the lymphatic progression of esophageal carcinoma.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Niigata University School of Medicine"}]},"item_7_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508361","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05677734","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kaneko, Kouji"}],"nameIdentifiers":[{"nameIdentifier":"51666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koyama, Yu"}],"nameIdentifiers":[{"nameIdentifier":"51667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanda, Tatsuo"}],"nameIdentifiers":[{"nameIdentifier":"51668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honma, Hideyuki"}],"nameIdentifiers":[{"nameIdentifier":"51669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohashi, Manabu"}],"nameIdentifiers":[{"nameIdentifier":"51670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakagawa, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"51671","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatakeyama, Katsuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"51672","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"110006483824.pdf","filesize":[{"value":"746.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110006483824.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/6209/files/110006483824.pdf"},"version_id":"41a2c19a-bca7-4ae8-81bc-871b66c42ce5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"VEGF-C","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF-D","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"esophageal carcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma"},{"subitem_title":"mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","579"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-09-28"},"publish_date":"2012-09-28","publish_status":"0","recid":"6209","relation_version_is_last":true,"title":["mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:39:35.312128+00:00"}