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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.55 No.3

Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells

http://hdl.handle.net/10191/20704
http://hdl.handle.net/10191/20704
9ed23f01-eb62-43b6-98f4-dbfc4bb0eb78
名前 / ファイル ライセンス アクション
110007138510.pdf 110007138510.pdf (551.3 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-01-21
タイトル
タイトル Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells
タイトル
言語 en
タイトル Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells
言語
言語 eng
キーワード
主題Scheme Other
主題 matrix metalloproteinase-2
キーワード
主題Scheme Other
主題 adenovirus vector
キーワード
主題Scheme Other
主題 trabecular meshwork
キーワード
主題Scheme Other
主題 primary open-angle glaucoma
キーワード
主題Scheme Other
主題 zymography
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Fukuchi, Takeo

× Fukuchi, Takeo

WEKO 51572

Fukuchi, Takeo

Search repository
Seki, Masaaki

× Seki, Masaaki

WEKO 51573

Seki, Masaaki

Search repository
Matsuda, Hidenobu

× Matsuda, Hidenobu

WEKO 51574

Matsuda, Hidenobu

Search repository
Ueda, Jun

× Ueda, Jun

WEKO 51575

Ueda, Jun

Search repository
Abe, Haruki

× Abe, Haruki

WEKO 51576

Abe, Haruki

Search repository
抄録
内容記述タイプ Abstract
内容記述 This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 55, 号 3, p. 81-86, 発行日 2012-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 13458485
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN1046766X
権利
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
著者版フラグ
値 publisher
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