{"_buckets": {"deposit": "aaa701ac-a5f4-4268-9a57-270a34dc9262"}, "_deposit": {"id": "6194", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6194"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006194"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "86", "bibliographicPageStart": "81", "bibliographicVolumeNumber": "55", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_rights_15": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u672c\u6587\u30c7\u30fc\u30bf\u306f\u5b66\u5354\u4f1a\u306e\u8a31\u8afe\u306b\u57fa\u3065\u304dCiNii\u304b\u3089\u8907\u88fd\u3057\u305f\u3082\u306e\u3067\u3042\u308b"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN1046766X", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13458485", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fukuchi, Takeo"}], "nameIdentifiers": [{"nameIdentifier": "51572", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seki, Masaaki"}], "nameIdentifiers": [{"nameIdentifier": "51573", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuda, Hidenobu"}], "nameIdentifiers": [{"nameIdentifier": "51574", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueda, Jun"}], "nameIdentifiers": [{"nameIdentifier": "51575", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Abe, Haruki"}], "nameIdentifiers": [{"nameIdentifier": "51576", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "110007138510.pdf", "filesize": [{"value": "551.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 551300.0, "url": {"label": "110007138510.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6194/files/110007138510.pdf"}, "version_id": "7082e771-6c47-4fda-86d5-bb3a37450b5e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "matrix metalloproteinase-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adenovirus vector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "trabecular meshwork", "subitem_subject_scheme": "Other"}, {"subitem_subject": "primary open-angle glaucoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "zymography", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells"}, {"subitem_title": "Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/575"], "permalink_uri": "http://hdl.handle.net/10191/20704", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-01-21"}, "publish_date": "2013-01-21", "publish_status": "0", "recid": "6194", "relation": {}, "relation_version_is_last": true, "title": ["Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells"], "weko_shared_id": null}