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Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells
http://hdl.handle.net/10191/20704
http://hdl.handle.net/10191/207049ed23f01-eb62-43b6-98f4-dbfc4bb0eb78
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
110007138510.pdf (551.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-01-21 | |||||
| タイトル | ||||||
| タイトル | Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells | |||||
| タイトル | ||||||
| タイトル | Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | matrix metalloproteinase-2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | adenovirus vector | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | trabecular meshwork | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | primary open-angle glaucoma | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | zymography | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Fukuchi, Takeo
× Fukuchi, Takeo× Seki, Masaaki× Matsuda, Hidenobu× Ueda, Jun× Abe, Haruki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 55, 号 3, p. 81-86, 発行日 2012-03 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13458485 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN1046766X | |||||
| 権利 | ||||||
| 権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Fukuchi, Takeo, Seki, Masaaki, Matsuda, Hidenobu, Ueda, Jun, Abe, Haruki, 2012, Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells: Niigata University School of Medicine, 81–86 p.
