{"created":"2021-03-01T06:10:05.221343+00:00","id":6194,"links":{},"metadata":{"_buckets":{"deposit":"aaa701ac-a5f4-4268-9a57-270a34dc9262"},"_deposit":{"id":"6194","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6194"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00006194","sets":["453:456","471:537:568:575"]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"86","bibliographicPageStart":"81","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"Acta medica et biologica"},{"bibliographic_title":"Acta medica et biologica","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Niigata University School of Medicine"}]},"item_7_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN1046766X","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13458485","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukuchi, Takeo"}],"nameIdentifiers":[{"nameIdentifier":"51572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seki, Masaaki"}],"nameIdentifiers":[{"nameIdentifier":"51573","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuda, Hidenobu"}],"nameIdentifiers":[{"nameIdentifier":"51574","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ueda, Jun"}],"nameIdentifiers":[{"nameIdentifier":"51575","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Haruki"}],"nameIdentifiers":[{"nameIdentifier":"51576","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"110007138510.pdf","filesize":[{"value":"551.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110007138510.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/6194/files/110007138510.pdf"},"version_id":"7082e771-6c47-4fda-86d5-bb3a37450b5e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"matrix metalloproteinase-2","subitem_subject_scheme":"Other"},{"subitem_subject":"adenovirus vector","subitem_subject_scheme":"Other"},{"subitem_subject":"trabecular meshwork","subitem_subject_scheme":"Other"},{"subitem_subject":"primary open-angle glaucoma","subitem_subject_scheme":"Other"},{"subitem_subject":"zymography","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells"},{"subitem_title":"Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","575"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-01-21"},"publish_date":"2013-01-21","publish_status":"0","recid":"6194","relation_version_is_last":true,"title":["Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:39:22.489857+00:00"}