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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation

http://hdl.handle.net/10191/48151
http://hdl.handle.net/10191/48151
588fa924-a6fd-4e6e-941d-6ec004b293d7
名前 / ファイル ライセンス アクション
h29nmk770-2.pdf 本文2 (700.2 kB)
h29nmk770-1.pdf 本文1 (952.0 kB)
h29nmk770_a.pdf 要旨 (216.4 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2017-11-07
タイトル
タイトル Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation
タイトル
言語 en
タイトル Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation
言語
言語 eng
キーワード
主題Scheme Other
主題 AGE
キーワード
主題Scheme Other
主題 BDNF
キーワード
主題Scheme Other
主題 Platelets
キーワード
主題Scheme Other
主題 Release
キーワード
主題Scheme Other
主題 Ca^<2+>
キーワード
主題Scheme Other
主題 Src family kinases
キーワード
主題Scheme Other
主題 Syk
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 最終糖化産物(AGEs)はヒト血小板において、Srcファミリーキナーゼの活性化を介して脳由来神経栄養因子(BDNF)の放出を促進させる
著者 Furukawa, Kazuo

× Furukawa, Kazuo

WEKO 51140

Furukawa, Kazuo

Search repository
著者別名
識別子 51141
識別子Scheme WEKO
姓名 古川, 和郎
抄録
内容記述タイプ Abstract
内容記述 Background: Brain-derived neurotrophic factor (BDNF) exerts beneficial effects not only on diabetic neuropathies but also on cardiovascular injury. There is argument regarding the levels of serum BDNF in patients with diabetes mellitus (DM). Because BDNF in peripheral blood is rich in platelets, this may represent dysregulation of BDNF release from platelets. Here we focused on advanced glycation end products (AGEs), which are elevated in patients with DM and have adverse effects on cardiovascular functions. The aim of this study is to elucidate the role of AGEs in the regulation of BDNF release from human platelets. Methods: Platelets collected from peripheral blood of healthy volunteers were incubated with various concentrations of AGE (glycated-BSA) at 37 °C for 5 min with or without BAPTA-AM, a cell permeable Ca^<2+> chelator, or PP2, a potent inhibitor of Src family kinases (SFKs). Released and cellular BDNF were measured by ELISA and calculated. Phosphorylation of Src and Syk, a downstream kinase of SFKs, in stimulated platelets was examined by Western blotting and immunoprecipitation. Results: AGE induced BDNF release from human platelets in a dose-dependent manner, which was dependent on intracellular Ca^<2+> and SFKs. We found that AGE induced phosphorylation of Src and Syk. Conclusions: AGE induces BDNF release from human platelets through the activation of the Src-Syk-(possibly phospholipase C)-Ca^<2+> pathway. Considering the toxic action of AGEs and the protective roles of BDNF, it can be hypothesized that AGE-induced BDNF release is a biological defense system in the early phase of diabetes. Chronic elevation of AGEs may induce depletion or downregulation of BDNF in platelets during the progression of DM.
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第4357号. 学位記番号: 新大院博(医)甲第770号. 学位授与年月日: 平成29年9月20日
内容記述
内容記述タイプ Other
内容記述 Cardiovascular Diabetology. 2017, 16, 20.
書誌情報 発行日 2017-09-20
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1186/s12933-017-0505-y
権利
権利情報 (C) The Author(s) 2017
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2017-09-20
学位授与番号
学位授与番号 13101甲第4357号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第770号
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