WEKO3
アイテム
Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation
http://hdl.handle.net/10191/48151
http://hdl.handle.net/10191/48151588fa924-a6fd-4e6e-941d-6ec004b293d7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文2 (700.2 kB)
|
|
|
|
本文1 (952.0 kB)
|
|
|
|
要旨 (216.4 kB)
|
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-11-07 | |||||
| タイトル | ||||||
| タイトル | Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation | |||||
| タイトル | ||||||
| タイトル | Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | AGE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | BDNF | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Platelets | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Release | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ca^<2+> | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Src family kinases | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Syk | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 最終糖化産物(AGEs)はヒト血小板において、Srcファミリーキナーゼの活性化を介して脳由来神経栄養因子(BDNF)の放出を促進させる | |||||
| 著者 |
Furukawa, Kazuo
× Furukawa, Kazuo |
|||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 51141 | |||||
| 姓名 | 古川, 和郎 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Brain-derived neurotrophic factor (BDNF) exerts beneficial effects not only on diabetic neuropathies but also on cardiovascular injury. There is argument regarding the levels of serum BDNF in patients with diabetes mellitus (DM). Because BDNF in peripheral blood is rich in platelets, this may represent dysregulation of BDNF release from platelets. Here we focused on advanced glycation end products (AGEs), which are elevated in patients with DM and have adverse effects on cardiovascular functions. The aim of this study is to elucidate the role of AGEs in the regulation of BDNF release from human platelets. Methods: Platelets collected from peripheral blood of healthy volunteers were incubated with various concentrations of AGE (glycated-BSA) at 37 °C for 5 min with or without BAPTA-AM, a cell permeable Ca^<2+> chelator, or PP2, a potent inhibitor of Src family kinases (SFKs). Released and cellular BDNF were measured by ELISA and calculated. Phosphorylation of Src and Syk, a downstream kinase of SFKs, in stimulated platelets was examined by Western blotting and immunoprecipitation. Results: AGE induced BDNF release from human platelets in a dose-dependent manner, which was dependent on intracellular Ca^<2+> and SFKs. We found that AGE induced phosphorylation of Src and Syk. Conclusions: AGE induces BDNF release from human platelets through the activation of the Src-Syk-(possibly phospholipase C)-Ca^<2+> pathway. Considering the toxic action of AGEs and the protective roles of BDNF, it can be hypothesized that AGE-induced BDNF release is a biological defense system in the early phase of diabetes. Chronic elevation of AGEs may induce depletion or downregulation of BDNF in platelets during the progression of DM. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 甲第4357号. 学位記番号: 新大院博（医）甲第770号. 学位授与年月日: 平成29年9月20日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Cardiovascular Diabetology. 2017, 16, 20. | |||||
| 書誌情報 | 発行日 2017-09-20 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1186/s12933-017-0505-y | |||||
| 権利 | ||||||
| 権利情報 | (C) The Author(s) 2017 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2017-09-20 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4357号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（医）甲第770号 | |||||
