{"created":"2021-03-01T06:09:49.960809+00:00","id":5953,"links":{},"metadata":{"_buckets":{"deposit":"65a9f035-66b2-44fc-8c2e-7c6ffa2c6aca"},"_deposit":{"id":"5953","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5953"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005953","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"最終糖化産物(AGEs)はヒト血小板において、Srcファミリーキナーゼの活性化を介して脳由来神経栄養因子(BDNF)の放出を促進させる"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09-20","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-09-20"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Brain-derived neurotrophic factor (BDNF) exerts beneficial effects not only on diabetic neuropathies but also on cardiovascular injury. There is argument regarding the levels of serum BDNF in patients with diabetes mellitus (DM). Because BDNF in peripheral blood is rich in platelets, this may represent dysregulation of BDNF release from platelets. Here we focused on advanced glycation end products (AGEs), which are elevated in patients with DM and have adverse effects on cardiovascular functions. The aim of this study is to elucidate the role of AGEs in the regulation of BDNF release from human platelets. Methods: Platelets collected from peripheral blood of healthy volunteers were incubated with various concentrations of AGE (glycated-BSA) at 37 °C for 5 min with or without BAPTA-AM, a cell permeable Ca^<2+> chelator, or PP2, a potent inhibitor of Src family kinases (SFKs). Released and cellular BDNF were measured by ELISA and calculated. Phosphorylation of Src and Syk, a downstream kinase of SFKs, in stimulated platelets was examined by Western blotting and immunoprecipitation. Results: AGE induced BDNF release from human platelets in a dose-dependent manner, which was dependent on intracellular Ca^<2+> and SFKs. We found that AGE induced phosphorylation of Src and Syk. Conclusions: AGE induces BDNF release from human platelets through the activation of the Src-Syk-(possibly phospholipase C)-Ca^<2+> pathway. Considering the toxic action of AGEs and the protective roles of BDNF, it can be hypothesized that AGE-induced BDNF release is a biological defense system in the early phase of diabetes. Chronic elevation of AGEs may induce depletion or downregulation of BDNF in platelets during the progression of DM.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 甲第4357号. 学位記番号: 新大院博（医）甲第770号. 学位授与年月日: 平成29年9月20日","subitem_description_type":"Other"},{"subitem_description":"Cardiovascular Diabetology. 2017, 16, 20.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（医）甲第770号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4357号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"51141","nameIdentifierScheme":"WEKO"}],"names":[{"name":"古川, 和郎"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1186/s12933-017-0505-y","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) The Author(s) 2017"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Furukawa, Kazuo"}],"nameIdentifiers":[{"nameIdentifier":"51140","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h29nmk770-2.pdf","filesize":[{"value":"700.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文2","url":"https://niigata-u.repo.nii.ac.jp/record/5953/files/h29nmk770-2.pdf"},"version_id":"e76e6b73-c34d-4456-ad16-a71c62088a83"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h29nmk770-1.pdf","filesize":[{"value":"952.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文1","url":"https://niigata-u.repo.nii.ac.jp/record/5953/files/h29nmk770-1.pdf"},"version_id":"0dd5defb-c147-437a-94ae-669d442b8ba2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h29nmk770_a.pdf","filesize":[{"value":"216.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5953/files/h29nmk770_a.pdf"},"version_id":"ad8e1acd-0bb5-491a-a9c2-ddf90bff8469"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AGE","subitem_subject_scheme":"Other"},{"subitem_subject":"BDNF","subitem_subject_scheme":"Other"},{"subitem_subject":"Platelets","subitem_subject_scheme":"Other"},{"subitem_subject":"Release","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca^<2+>","subitem_subject_scheme":"Other"},{"subitem_subject":"Src family kinases","subitem_subject_scheme":"Other"},{"subitem_subject":"Syk","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation"},{"subitem_title":"Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-07"},"publish_date":"2017-11-07","publish_status":"0","recid":"5953","relation_version_is_last":true,"title":["Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:39:13.392619+00:00"}