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MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such as cytokines.Little is known about the role of interferon (IFN)-γ in MUC5AC expression in human bronchial epithelial cells. Methods: Human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) and normal human bronchial epithelial (NHBE) cells were used to assess the effects of IFN-γ on MUC5AC transcription. Results: Transforming growth factor (TGF)-α and double-stranded RNA (polyI:C)-induced MUC5AC mRNA and protein expression was repressed by IFN-γin a concentration-dependent manner. IFN-γ showed limited effects on TGF-α and polyI:C-induced activation of epidermal growth factor receptor (EGFR) and extracellular signale-regulated kinase(ERK). 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Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ
http://hdl.handle.net/10191/45098
http://hdl.handle.net/10191/45098a71c5ac4-fa07-4633-9176-5f84576a3d52
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-12-07 | |||||
タイトル | ||||||
タイトル | Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MUC5AC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mucin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interferon-ɤ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bronchial epithelial cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sp1 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | インターフェロンγによるヒト気道上皮細胞MUC5AC発現抑制 | |||||
著者 |
Oyanagi, Takahito
× Oyanagi, Takahito |
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著者別名 | ||||||
識別子 | 50901 | |||||
識別子Scheme | WEKO | |||||
姓名 | 小柳, 貴人 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Excessive mucin secretion in the airway is an important feature of airway inflammatory diseases. . MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such as cytokines.Little is known about the role of interferon (IFN)-γ in MUC5AC expression in human bronchial epithelial cells. Methods: Human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) and normal human bronchial epithelial (NHBE) cells were used to assess the effects of IFN-γ on MUC5AC transcription. Results: Transforming growth factor (TGF)-α and double-stranded RNA (polyI:C)-induced MUC5AC mRNA and protein expression was repressed by IFN-γin a concentration-dependent manner. IFN-γ showed limited effects on TGF-α and polyI:C-induced activation of epidermal growth factor receptor (EGFR) and extracellular signale-regulated kinase(ERK). A chromatin immunoprecipitation assay indicated that Sp1 bound to its cognate sequence located on the MUC5AC promoter. The Sp1inhibitor mithramycin A inhibited MUC5AC mRNA expression, implying a critical role for Sp1 in MUC5AC induction. Importantly, IFN-γ impeded Sp1 binding to the MUC5AC promoter. Conclusions: These results suggest that IFN-γ represses MUC5AC expression, disturbing binding of Sp1 to its target sequences. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 学位の種類: 博士(医学). 報告番号: 甲第4212号. 学位記番号: 新大院博(医)甲第711号. 学位授与年月日: 平成28年9月20日 | |||||
書誌情報 | 発行日 2016-09-20 | |||||
出版者 | ||||||
出版者 | 新潟大学 | |||||
著者版フラグ | ||||||
値 | ETD | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-09-20 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4212号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(医)甲第711号 |