{"_buckets": {"deposit": "1d50e44b-f669-469e-90f9-2cec0ed86e52"}, "_deposit": {"id": "5800", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5800"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00005800", "sets": ["455", "562"]}, "item_6_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "インターフェロンγによるヒト気道上皮細胞MUC5AC発現抑制"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-09-20", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2016-09-20"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "新潟大学"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Excessive mucin secretion in the airway is an important feature of airway inflammatory diseases. . MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such as cytokines.Little is known about the role of interferon (IFN)-γ in MUC5AC expression in human bronchial epithelial cells. Methods: Human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) and normal human bronchial epithelial (NHBE) cells were used to assess the effects of IFN-γ on MUC5AC transcription. Results: Transforming growth factor (TGF)-α and double-stranded RNA (polyI:C)-induced MUC5AC mRNA and protein expression was repressed by IFN-γin a concentration-dependent manner. IFN-γ showed limited effects on TGF-α and polyI:C-induced activation of epidermal growth factor receptor (EGFR) and extracellular signale-regulated kinase(ERK). A chromatin immunoprecipitation assay indicated that Sp1 bound to its cognate sequence located on the MUC5AC promoter. The Sp1inhibitor mithramycin A inhibited MUC5AC mRNA expression, implying a critical role for Sp1 in MUC5AC induction. Importantly, IFN-γ impeded Sp1 binding to the MUC5AC promoter. Conclusions: These results suggest that IFN-γ represses MUC5AC expression,  disturbing binding of Sp1  to its target sequences.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "学位の種類: 博士（医学）. 報告番号: 甲第4212号. 学位記番号: 新大院博（医）甲第711号. 学位授与年月日: 平成28年9月20日", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博（医）甲第711号", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101甲第4212号"}]}, "item_6_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "50901", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "小柳, 貴人"}]}]}, "item_6_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学"}]}, "item_6_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Oyanagi, Takahito"}], "nameIdentifiers": [{"nameIdentifier": "50900", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "h28nmk711.pdf", "filesize": [{"value": "6.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 6400000.0, "url": {"label": "本文", "url": "https://niigata-u.repo.nii.ac.jp/record/5800/files/h28nmk711.pdf"}, "version_id": "538406ed-8686-4f8f-8495-b74b8072aa6e"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "h28nmk711_a.pdf", "filesize": [{"value": "209.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 209200.0, "url": {"label": "要旨", "url": "https://niigata-u.repo.nii.ac.jp/record/5800/files/h28nmk711_a.pdf"}, "version_id": "b35b4747-da77-40a9-a3e7-6952e41c70eb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "MUC5AC", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mucin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interferon-ɤ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "bronchial epithelial cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sp1", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ"}, {"subitem_title": "Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "562"], "permalink_uri": "http://hdl.handle.net/10191/45098", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-12-07"}, "publish_date": "2016-12-07", "publish_status": "0", "recid": "5800", "relation": {}, "relation_version_is_last": true, "title": ["Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ"], "weko_shared_id": 2}