{"created":"2021-03-01T06:09:40.338635+00:00","id":5800,"links":{},"metadata":{"_buckets":{"deposit":"1d50e44b-f669-469e-90f9-2cec0ed86e52"},"_deposit":{"id":"5800","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5800"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005800","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"インターフェロンγによるヒト気道上皮細胞MUC5AC発現抑制"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-20","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-09-20"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Excessive mucin secretion in the airway is an important feature of airway inflammatory diseases. . MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such MUC5AC expression is regulated by a variety of stimuli such as cytokines.Little is known about the role of interferon (IFN)-γ in MUC5AC expression in human bronchial epithelial cells. Methods: Human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) and normal human bronchial epithelial (NHBE) cells were used to assess the effects of IFN-γ on MUC5AC transcription. Results: Transforming growth factor (TGF)-α and double-stranded RNA (polyI:C)-induced MUC5AC mRNA and protein expression was repressed by IFN-γin a concentration-dependent manner. IFN-γ showed limited effects on TGF-α and polyI:C-induced activation of epidermal growth factor receptor (EGFR) and extracellular signale-regulated kinase(ERK). A chromatin immunoprecipitation assay indicated that Sp1 bound to its cognate sequence located on the MUC5AC promoter. The Sp1inhibitor mithramycin A inhibited MUC5AC mRNA expression, implying a critical role for Sp1 in MUC5AC induction. Importantly, IFN-γ impeded Sp1 binding to the MUC5AC promoter. Conclusions: These results suggest that IFN-γ represses MUC5AC expression,  disturbing binding of Sp1  to its target sequences.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 甲第4212号. 学位記番号: 新大院博（医）甲第711号. 学位授与年月日: 平成28年9月20日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（医）甲第711号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4212号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50901","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小柳, 貴人"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oyanagi, Takahito"}],"nameIdentifiers":[{"nameIdentifier":"50900","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h28nmk711.pdf","filesize":[{"value":"6.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/5800/files/h28nmk711.pdf"},"version_id":"538406ed-8686-4f8f-8495-b74b8072aa6e"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h28nmk711_a.pdf","filesize":[{"value":"209.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5800/files/h28nmk711_a.pdf"},"version_id":"b35b4747-da77-40a9-a3e7-6952e41c70eb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MUC5AC","subitem_subject_scheme":"Other"},{"subitem_subject":"mucin","subitem_subject_scheme":"Other"},{"subitem_subject":"interferon-ɤ","subitem_subject_scheme":"Other"},{"subitem_subject":"bronchial epithelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Sp1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ"},{"subitem_title":"Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-12-07"},"publish_date":"2016-12-07","publish_status":"0","recid":"5800","relation_version_is_last":true,"title":["Suppression of MUC5AC expression in human bronchial epithelial cells by interferon-γ"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:39:01.449148+00:00"}