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Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells
http://hdl.handle.net/10191/23707
http://hdl.handle.net/10191/2370716774829-bce9-47a7-8835-8279de12115c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (6.3 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2013-10-09 | |||||
| タイトル | ||||||
| タイトル | Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells | |||||
| タイトル | ||||||
| タイトル | Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | マウス胚性幹細胞を用いて樹立されたジーントラップ系統マウスの研究 | |||||
| 著者 |
Miyashita, Akinori
× Miyashita, Akinori |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 48480 | |||||
| 姓名 | 宮下, 哲典 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We characterized a region of the mouse genome disrupted by integration of a gene trap (GT) vector in ES cells. On 5 rapid amplification of cDNA ends analysis of the fusion transcripts containing the GT vector we identified the eukaryotic protein synthesis initiation factor 4A1 gene (Eif4a1) as a promoter-trapped gene. Plasmid rescue was used to show that the other end of the integrated vector disrupted the murine homolog of the human fragile X mental retardation syndrome-related protein 2 gene (Fxr2h). Structural analysis of P1 clones, isolated from the wild type mouse genome by PCR with Eif4a1 specific primers, indicated that the integration of the GT vector was accompanied by the deletion of about 35 kb of genomic DNA and that the disrupted region also included three genes, Cd68, Sup115h and Sox15, the latter two if which are transcribed in opposite directions with overlapping 3 ends. These five different genes at least, Eif4a1, Cd68, Sup115h, Sox15 and Fxr2h, are clustered in a 40 kb region. The chromosomal location of this region was mapped by means of interspecific backcross panel DNAs to the central part of mouse chromosome 11, exhibiting a known region of synteny with human chromosome 17. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（理学）. 報告番号: 甲第1643号. 学位記番号: 新大院博（理）甲第135号. 学位授与年月日: 平成12年3月24日 | |||||
| 書誌情報 | p. 1-61, 発行日 2000-03-24 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2000-03-24 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101A1643 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（理）甲第135号 | |||||
