{"created":"2021-03-01T06:08:15.950524+00:00","id":4462,"links":{},"metadata":{"_buckets":{"deposit":"955731df-0eb1-486f-90a5-2b3113b47ecd"},"_deposit":{"id":"4462","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4462"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00004462","sets":["453:455","468:563:564"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"マウス胚性幹細胞を用いて樹立されたジーントラップ系統マウスの研究"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-03-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"61","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2000-03-24"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We characterized a region of the mouse genome disrupted by integration of a gene trap (GT) vector in ES cells. On 5 rapid amplification of cDNA ends analysis of the fusion transcripts containing the GT vector we identified the eukaryotic protein synthesis initiation factor 4A1 gene (Eif4a1) as a promoter-trapped gene. Plasmid rescue was used to show that the other end of the integrated vector disrupted the murine homolog of the human fragile X mental retardation syndrome-related protein 2 gene (Fxr2h). Structural analysis of P1 clones, isolated from the wild type mouse genome by PCR with Eif4a1 specific primers, indicated that the integration of the GT vector was accompanied by the deletion of about 35 kb of genomic DNA and that the disrupted region also included three genes, Cd68, Sup115h and Sox15, the latter two if which are transcribed in opposite directions with overlapping 3 ends. These five different genes at least, Eif4a1, Cd68, Sup115h, Sox15 and Fxr2h, are clustered in a 40 kb region. The chromosomal location of this region was mapped by means of interspecific backcross panel DNAs to the central part of mouse chromosome 11, exhibiting a known region of synteny with human chromosome 17.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（理学）. 報告番号: 甲第1643号. 学位記番号: 新大院博（理）甲第135号. 学位授与年月日: 平成12年3月24日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（理）甲第135号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101A1643"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"48480","nameIdentifierScheme":"WEKO"}],"names":[{"name":"宮下, 哲典"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyashita, Akinori"}],"nameIdentifiers":[{"nameIdentifier":"48479","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"000000358474.pdf","filesize":[{"value":"6.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/4462/files/000000358474.pdf"},"version_id":"1f53f908-0acb-404f-9cea-82a41620e206"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells"},{"subitem_title":"Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","564"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-09"},"publish_date":"2013-10-09","publish_status":"0","recid":"4462","relation_version_is_last":true,"title":["Study of the Mutant Mice Produced by Gene Trapping Using Mouse Embryonic Stem Cells"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:37:21.791396+00:00"}