WEKO3
アイテム
{"_buckets": {"deposit": "7c3bd0b3-aec9-4659-a7fd-86d0142207e6"}, "_deposit": {"id": "34161", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "34161"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00034161", "sets": ["455", "562"]}, "item_6_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "無機のポリリン酸はリポ多糖がマクロファージに引き起こす炎症反応を増強する"}]}, "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2020-09-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "新潟大学"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate units that are linked by phosphoanhydride bonds and is involved in various pathophysiological processes. However, the role of polyP in immune cell dysfunction is not well-understood. In this study, using several biochemical and cell biology approaches, including cytokine assays, immunofluorescence microscopy, receptor-binding assays with quartz crystal microbalance, and dynamic light scanning, we investigated the effect of polyP on in vitro lipopolysaccharide (LPS)- induced macrophage inflammatory response. PolyP up-regulated LPS-induced production of the inflammatory cytokines, such as tumor necrosis factor , interleukin-1, and interleukin-6, in macrophages, and the effect was polyP dose– and chain length– dependent. However, orthophosphate did not exhibit this effect. PolyP enhanced the LPS-induced intracellular macrophage inflammatory signals. Affinity analysis revealed that polyP interacts with LPS, inducing formation of small micelles, and the polyP-LPS complex enhanced the binding affinity of LPS to Toll-like receptor 4 (TLR4) on macrophages. These results suggest that inorganic polyP plays a critical role in promoting inflammatory response by enhancing the interaction between LPS and TLR4 in macrophages.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Journal of Biological Chemistry. 2020, 295(12), 4014-4023.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博(医)甲第968号", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101甲第4798号"}]}, "item_6_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "178225", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "伊藤, 徹"}]}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1074/jbc.RA119.011763", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 2020 Ito et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an open access article under the CC BY-NC-SA license (https://creativecommons.org/licenses/by-nc-sa/4.0/)."}]}, "item_6_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ito, Toru"}], "nameIdentifiers": [{"nameIdentifier": "178224", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r2nmk968.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "本文", "url": "https://niigata-u.repo.nii.ac.jp/record/34161/files/r2nmk968.pdf"}, "version_id": "55666012-a894-442b-b9ea-cb6a8bbd4141"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "r2nmk968_a.pdf", "filesize": [{"value": "455.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 455700.0, "url": {"label": "要旨", "url": "https://niigata-u.repo.nii.ac.jp/record/34161/files/r2nmk968_a.pdf"}, "version_id": "674e13b4-7356-4193-9cd2-b5fda2d5f62c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "562"], "permalink_uri": "http://hdl.handle.net/10191/00051959", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-11-12"}, "publish_date": "2020-11-12", "publish_status": "0", "recid": "34161", "relation": {}, "relation_version_is_last": true, "title": ["Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response"], "weko_shared_id": null}
Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response
http://hdl.handle.net/10191/00051959
http://hdl.handle.net/10191/000519595932d403-ab45-4e9f-bd21-4e8f712d3869
名前 / ファイル | ライセンス | アクション |
---|---|---|
本文 (2.8 MB)
|
|
|
要旨 (455.7 kB)
|
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-11-12 | |||||
タイトル | ||||||
タイトル | Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | 無機のポリリン酸はリポ多糖がマクロファージに引き起こす炎症反応を増強する | |||||
著者 |
Ito, Toru
× Ito, Toru |
|||||
著者別名 | ||||||
識別子 | 178225 | |||||
識別子Scheme | WEKO | |||||
姓名 | 伊藤, 徹 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate units that are linked by phosphoanhydride bonds and is involved in various pathophysiological processes. However, the role of polyP in immune cell dysfunction is not well-understood. In this study, using several biochemical and cell biology approaches, including cytokine assays, immunofluorescence microscopy, receptor-binding assays with quartz crystal microbalance, and dynamic light scanning, we investigated the effect of polyP on in vitro lipopolysaccharide (LPS)- induced macrophage inflammatory response. PolyP up-regulated LPS-induced production of the inflammatory cytokines, such as tumor necrosis factor , interleukin-1, and interleukin-6, in macrophages, and the effect was polyP dose– and chain length– dependent. However, orthophosphate did not exhibit this effect. PolyP enhanced the LPS-induced intracellular macrophage inflammatory signals. Affinity analysis revealed that polyP interacts with LPS, inducing formation of small micelles, and the polyP-LPS complex enhanced the binding affinity of LPS to Toll-like receptor 4 (TLR4) on macrophages. These results suggest that inorganic polyP plays a critical role in promoting inflammatory response by enhancing the interaction between LPS and TLR4 in macrophages. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Biological Chemistry. 2020, 295(12), 4014-4023. | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1074/jbc.RA119.011763 | |||||
権利 | ||||||
権利情報 | 【○!C】 2020 Ito et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an open access article under the CC BY-NC-SA license (https://creativecommons.org/licenses/by-nc-sa/4.0/). | |||||
著者版フラグ | ||||||
値 | ETD | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-09-23 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4798号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(医)甲第968号 |