{"created":"2021-03-01T06:40:37.700886+00:00","id":34161,"links":{},"metadata":{"_buckets":{"deposit":"7c3bd0b3-aec9-4659-a7fd-86d0142207e6"},"_deposit":{"id":"34161","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34161"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00034161"},"item_6_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"\u7121\u6a5f\u306e\u30dd\u30ea\u30ea\u30f3\u9178\u306f\u30ea\u30dd\u591a\u7cd6\u304c\u30de\u30af\u30ed\u30d5\u30a1\u30fc\u30b8\u306b\u5f15\u304d\u8d77\u3053\u3059\u708e\u75c7\u53cd\u5fdc\u3092\u5897\u5f37\u3059\u308b"}]},"item_6_date_granted_51":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5","attribute_value_mlt":[{"subitem_dategranted":"2020-09-23"}]},"item_6_degree_grantor_49":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"\u65b0\u6f5f\u5927\u5b66"}]}]},"item_6_degree_name_48":{"attribute_name":"\u5b66\u4f4d\u540d","attribute_value_mlt":[{"subitem_degreename":"\u535a\u58eb(\u533b\u5b66)"}]},"item_6_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate units that are linked by phosphoanhydride bonds and is involved in various pathophysiological processes. However, the role of polyP in immune cell dysfunction is not well-understood. In this study, using several biochemical and cell biology approaches, including cytokine assays, immunofluorescence microscopy, receptor-binding assays with quartz crystal microbalance, and dynamic light scanning, we investigated the effect of polyP on in vitro lipopolysaccharide (LPS)- induced macrophage inflammatory response. PolyP up-regulated LPS-induced production of the inflammatory cytokines, such as tumor necrosis factor , interleukin-1, and interleukin-6, in macrophages, and the effect was polyP dose\u2013 and chain length\u2013 dependent. However, orthophosphate did not exhibit this effect. PolyP enhanced the LPS-induced intracellular macrophage inflammatory signals. Affinity analysis revealed that polyP interacts with LPS, inducing formation of small micelles, and the polyP-LPS complex enhanced the binding affinity of LPS to Toll-like receptor 4 (TLR4) on macrophages. These results suggest that inorganic polyP plays a critical role in promoting inflammatory response by enhancing the interaction between LPS and TLR4 in macrophages.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"\u5185\u5bb9\u8a18\u8ff0","attribute_value_mlt":[{"subitem_description":"Journal of Biological Chemistry. 2020, 295(12), 4014-4023.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"\u5b66\u4f4d\u8a18\u756a\u53f7","attribute_value_mlt":[{"subitem_description":"\u65b0\u5927\u9662\u535a(\u533b)\u7532\u7b2c968\u53f7","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7","attribute_value_mlt":[{"subitem_dissertationnumber":"13101\u7532\u7b2c4798\u53f7"}]},"item_6_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"178225","nameIdentifierScheme":"WEKO"}],"names":[{"name":"\u4f0a\u85e4, \u5fb9"}]}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1074/jbc.RA119.011763","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"\u6a29\u5229","attribute_value_mlt":[{"subitem_rights":"\u3010\u25cb!C\u3011 2020 Ito et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an open access article under the CC BY-NC-SA license (https://creativecommons.org/licenses/by-nc-sa/4.0/)."}]},"item_6_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ito, Toru"}],"nameIdentifiers":[{"nameIdentifier":"178224","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-12"}],"displaytype":"detail","filename":"r2nmk968.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"\u672c\u6587","url":"https://niigata-u.repo.nii.ac.jp/record/34161/files/r2nmk968.pdf"},"version_id":"55666012-a894-442b-b9ea-cb6a8bbd4141"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-12"}],"displaytype":"detail","filename":"r2nmk968_a.pdf","filesize":[{"value":"455.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"\u8981\u65e8","url":"https://niigata-u.repo.nii.ac.jp/record/34161/files/r2nmk968_a.pdf"},"version_id":"674e13b4-7356-4193-9cd2-b5fda2d5f62c"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response"}]},"item_type_id":"6","owner":"1","path":["453/455","471/561/562"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2020-11-12"},"publish_date":"2020-11-12","publish_status":"0","recid":"34161","relation_version_is_last":true,"title":["Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T07:50:10.240226+00:00"}