{"_buckets": {"deposit": "788dea52-5457-4b7f-8cb9-8f8987d8ad75"}, "_deposit": {"id": "28475", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28475"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028475", "sets": ["456", "1607"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "イネWx遺伝子の胚乳特異的サイレンシングを引き起こすイントロンループ型Wx 5\u0027UTR RNAiベクター"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "128", "bibliographicPageStart": "121", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To evoke endosperm specific disruption of gene function and quantitatively regulated RNAi, ihp dsRNA vectors, pWRI-A and pWRI-B, were constructed by using endosperm specific promoter and 1st intron of Wx gene. pWRI-B carries a single mutation at the 5\u0027 splicing site of 1st intron and the mutation reduces the mature transcript of the dsRNA gene. Both vectors efficiently disrupted Wx mRNA function among 96-100% of transgenic rice, and the degrees of Wx gene suppression at WRI-B/Wxb endosperms were weaker than that of WRI-A/Wxb endosperms. These results clearly showed the Wx promoter regulates endosperm specific RNAi. Moreover, the controlling the splicing efficiency can quantitatively regulates the suppression effect on RNAi.胚乳特異的な遺伝子機能の破壊及びRNAiの量的調節について解明するため、胚乳特異的プロモーターで誘導され、第一イントロンをループに持つRNAiベクター、pWRI-A、pWRI-Bを構築した。pWRI-Bは第一イントロンの5\u0027側スプライシング部位に塩基置換があり、RNAi特異的二本鎖RNAの蓄積量が減少する。両方のRNAiベクターとも96～100%の形質転換体で内在Wx mRNAの発現抑制が観察された。さらに、WRI-B/Wxbの抑制効果はWRI-A/Wxbよりも弱かった。これらの結果により、Wxプロモーターが胚乳特異的なRNAiを誘導させることが明らかとなった。さらに、スプライス効率の調節がRNAiの抑制効果を制御できる可能性が示唆された。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163802", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "丹野, 史典"}]}, {"nameIdentifiers": [{"nameIdentifier": "163803", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "尾崎, 寛子"}]}, {"nameIdentifiers": [{"nameIdentifier": "163804", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "伊藤, 紀美子"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tanno, Fuminori"}], "nameIdentifiers": [{"nameIdentifier": "163799", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ozaki, Hiroko"}], "nameIdentifiers": [{"nameIdentifier": "163800", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163801", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "17_0018.pdf", "filesize": [{"value": "285.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 285000.0, "url": {"label": "17_0018.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28475/files/17_0018.pdf"}, "version_id": "42e63da9-cf34-4d83-b7d8-d8ea5d120d45"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "RNA interference", "subitem_subject_scheme": "Other"}, {"subitem_subject": "splicing efficiency", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oryza sativa L.", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Granule-bound starch synthase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Waxy", "subitem_subject_scheme": "Other"}, {"subitem_subject": "siRNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RNA干渉", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "スプライシング効率", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "イネ", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "デンプン合成酵素", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "siRNA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"}, {"subitem_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1607"], "permalink_uri": "http://hdl.handle.net/10191/675", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28475", "relation": {}, "relation_version_is_last": true, "title": ["Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"], "weko_shared_id": null}