{"_buckets": {"deposit": "788dea52-5457-4b7f-8cb9-8f8987d8ad75"}, "_deposit": {"id": "28475", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28475"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028475"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a4\u30cdWx\u907a\u4f1d\u5b50\u306e\u80da\u4e73\u7279\u7570\u7684\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u3092\u5f15\u304d\u8d77\u3053\u3059\u30a4\u30f3\u30c8\u30ed\u30f3\u30eb\u30fc\u30d7\u578bWx 5\u0027UTR RNAi\u30d9\u30af\u30bf\u30fc"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "128", "bibliographicPageStart": "121", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "To evoke endosperm specific disruption of gene function and quantitatively regulated RNAi, ihp dsRNA vectors, pWRI-A and pWRI-B, were constructed by using endosperm specific promoter and 1st intron of Wx gene. pWRI-B carries a single mutation at the 5\u0027 splicing site of 1st intron and the mutation reduces the mature transcript of the dsRNA gene. Both vectors efficiently disrupted Wx mRNA function among 96-100% of transgenic rice, and the degrees of Wx gene suppression at WRI-B/Wxb endosperms were weaker than that of WRI-A/Wxb endosperms. These results clearly showed the Wx promoter regulates endosperm specific RNAi. 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"subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30c7\u30f3\u30d7\u30f3\u5408\u6210\u9175\u7d20", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "siRNA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"}, {"subitem_title": "Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1607"], "permalink_uri": "http://hdl.handle.net/10191/675", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28475", "relation": {}, "relation_version_is_last": true, "title": ["Intron Loop Wx 5\u0027UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"], "weko_shared_id": null}