{"created":"2021-03-01T06:34:17.479714+00:00","id":28475,"links":{},"metadata":{"_buckets":{"deposit":"788dea52-5457-4b7f-8cb9-8f8987d8ad75"},"_deposit":{"id":"28475","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28475"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028475","sets":["453:456","485:872:1583:1607"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"イネWx遺伝子の胚乳特異的サイレンシングを引き起こすイントロンループ型Wx 5'UTR RNAiベクター"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"128","bibliographicPageStart":"121","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To evoke endosperm specific disruption of gene function and quantitatively regulated RNAi, ihp dsRNA vectors, pWRI-A and pWRI-B, were constructed by using endosperm specific promoter and 1st intron of Wx gene. pWRI-B carries a single mutation at the 5' splicing site of 1st intron and the mutation reduces the mature transcript of the dsRNA gene. Both vectors efficiently disrupted Wx mRNA function among 96-100% of transgenic rice, and the degrees of Wx gene suppression at WRI-B/Wxb endosperms were weaker than that of WRI-A/Wxb endosperms. These results clearly showed the Wx promoter regulates endosperm specific RNAi. Moreover, the controlling the splicing efficiency can quantitatively regulates the suppression effect on RNAi.胚乳特異的な遺伝子機能の破壊及びRNAiの量的調節について解明するため、胚乳特異的プロモーターで誘導され、第一イントロンをループに持つRNAiベクター、pWRI-A、pWRI-Bを構築した。pWRI-Bは第一イントロンの5'側スプライシング部位に塩基置換があり、RNAi特異的二本鎖RNAの蓄積量が減少する。両方のRNAiベクターとも96～100%の形質転換体で内在Wx mRNAの発現抑制が観察された。さらに、WRI-B/Wxbの抑制効果はWRI-A/Wxbよりも弱かった。これらの結果により、Wxプロモーターが胚乳特異的なRNAiを誘導させることが明らかとなった。さらに、スプライス効率の調節がRNAiの抑制効果を制御できる可能性が示唆された。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163802","nameIdentifierScheme":"WEKO"}],"names":[{"name":"丹野, 史典"}]},{"nameIdentifiers":[{"nameIdentifier":"163803","nameIdentifierScheme":"WEKO"}],"names":[{"name":"尾崎, 寛子"}]},{"nameIdentifiers":[{"nameIdentifier":"163804","nameIdentifierScheme":"WEKO"}],"names":[{"name":"伊藤, 紀美子"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanno, Fuminori"}],"nameIdentifiers":[{"nameIdentifier":"163799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ozaki, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"163800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Kimiko"}],"nameIdentifiers":[{"nameIdentifier":"163801","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"17_0018.pdf","filesize":[{"value":"285.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17_0018.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28475/files/17_0018.pdf"},"version_id":"42e63da9-cf34-4d83-b7d8-d8ea5d120d45"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RNA interference","subitem_subject_scheme":"Other"},{"subitem_subject":"splicing efficiency","subitem_subject_scheme":"Other"},{"subitem_subject":"Oryza sativa L.","subitem_subject_scheme":"Other"},{"subitem_subject":"Granule-bound starch synthase","subitem_subject_scheme":"Other"},{"subitem_subject":"Waxy","subitem_subject_scheme":"Other"},{"subitem_subject":"siRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA干渉","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"スプライシング効率","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"イネ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"デンプン合成酵素","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intron Loop Wx 5'UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intron Loop Wx 5'UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"},{"subitem_title":"Intron Loop Wx 5'UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1607"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-18"},"publish_date":"2007-04-18","publish_status":"0","recid":"28475","relation_version_is_last":true,"title":["Intron Loop Wx 5'UTR dsRNA Vectors Mediated Endosperm Specific Silencing of Wx Gene of Rice"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:37.171621+00:00"}