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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第58巻第2号

Identification of Differentially Expressed Genes in Thai Native and Broiler Chicken Muscles

http://hdl.handle.net/10191/689
http://hdl.handle.net/10191/689
f3a8c9b6-1528-4ec9-9651-cf3cd9fc1e50
名前 / ファイル ライセンス アクション
17_0044.pdf 17_0044.pdf (104.7 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2007-04-18
タイトル
タイトル Identification of Differentially Expressed Genes in Thai Native and Broiler Chicken Muscles
タイトル
タイトル Identification of Differentially Expressed Genes in Thai Native and Broiler Chicken Muscles
言語 en
言語
言語 eng
キーワード
主題Scheme Other
主題 Thai native chicken
キーワード
主題Scheme Other
主題 broiler
キーワード
主題Scheme Other
主題 DDRT-PCR
キーワード
主題Scheme Other
主題 gene expression
キーワード
主題Scheme Other
主題 pectoralis muscle
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Supamit, Mekchay

× Supamit, Mekchay

WEKO 163688

Supamit, Mekchay

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Petai, Pongpaichan

× Petai, Pongpaichan

WEKO 163689

Petai, Pongpaichan

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Amnuay, Leotaragul

× Amnuay, Leotaragul

WEKO 163690

Amnuay, Leotaragul

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Patcharin, Krutmuang

× Patcharin, Krutmuang

WEKO 163691

Patcharin, Krutmuang

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抄録
内容記述タイプ Abstract
内容記述 Differential gene expression profiling in broiler and Thai native chicken muscle was analysed by using mRNA differential display reverse transcription-PCR (DDRT-PCR) technique. Total RNA was isolated from the pectoralis (breast) muscles at 1, 2, 4 and 6 weeks-age and 8 RNA pools were prepared based on 5 individual intra-breeds with the same age. Differential display banding patterns were produced using two anchor primers dT12VC (V:A, C, G) or dT12VG and 26 arbitrary primers. Comparing the banding patterns of the broiler and native chicken muscles revealed 7 differential markers from 5 primer combinations. In this investigation, the profiling of differentially expression cDNA bands could be divided into 4 categories: (i) bands only detected in broilers (all weeks-age); (ii) bands only detected in the native chickens (all weeks-age); (iii) bands only absent at 1 week-age in both chickens and (iv) bands only absent at 4 and 6 weeks-age of broilers. In category i and ii represented the gene expression profiling in muscle associated with the specific chicken breeds. In category iii and iv represented the transcriptional change upregulated and downregulated genes which associated with aging of chickens, respectively. We have successfully applied DDRT-PCR to identify the differential gene expression pro filing in broiler and Thai native chicken muscles. Further characterization of these differentially expression cDNA bands (consist of reamplification, cloning and sequencing as well as quantitative PCR analysis) provides the important candidate genes for meat quality in chicken.
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 58, 号 2, p. 147-150, 発行日 2006-03
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
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Cite as

Supamit, Mekchay, Petai, Pongpaichan, Amnuay, Leotaragul, Patcharin, Krutmuang, 2006, Identification of Differentially Expressed Genes in Thai Native and Broiler Chicken Muscles: 新潟大学農学部, 147–150 p.

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