@article{oai:niigata-u.repo.nii.ac.jp:00028454,
 author = {Supamit, Mekchay and Petai, Pongpaichan and Amnuay, Leotaragul and Patcharin, Krutmuang},
 issue = {2},
 journal = {新潟大学農学部研究報告, 新潟大学農学部研究報告},
 month = {Mar},
 note = {Differential gene expression profiling in broiler and Thai native chicken muscle was analysed by using mRNA differential display reverse transcription-PCR (DDRT-PCR) technique. Total RNA was isolated from the pectoralis (breast) muscles at 1, 2, 4 and 6 weeks-age and 8 RNA pools were prepared based on 5 individual intra-breeds with the same age. Differential display banding patterns were produced using two anchor primers dT12VC (V:A, C, G) or dT12VG and 26 arbitrary primers. Comparing the banding patterns of the broiler and native chicken muscles revealed 7 differential markers from 5 primer combinations. In this investigation, the profiling of differentially expression cDNA bands could be divided into 4 categories: (i) bands only detected in broilers (all weeks-age); (ii) bands only detected in the native chickens (all weeks-age); (iii) bands only absent at 1 week-age in both chickens and (iv) bands only absent at 4 and 6 weeks-age of broilers. In category i and ii represented the gene expression profiling in muscle associated with the specific chicken breeds. In category iii and iv represented the transcriptional change upregulated and downregulated genes which associated with aging of chickens, respectively. We have successfully applied DDRT-PCR to identify the differential gene expression pro filing in broiler and Thai native chicken muscles. Further characterization of these differentially expression cDNA bands (consist of reamplification, cloning and sequencing as well as quantitative PCR analysis) provides the important candidate genes for meat quality in chicken.},
 pages = {147--150},
 title = {Identification of Differentially Expressed Genes in Thai Native and Broiler Chicken Muscles},
 volume = {58},
 year = {2006}
}