{"_buckets": {"deposit": "030380d5-a503-4c78-910a-96f62fad480a"}, "_deposit": {"id": "28421", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28421"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028421", "sets": ["456", "1602"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "根こぶ病抵抗性カブ（Brassica rapa L.）培養根へのFura-2の導入と細胞内Ca2+ 濃度の測定"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "66", "bibliographicPageStart": "61", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Our previous study using liquid organ cultured adventitious roots from turnip (Brassica rapa L.) showed that Ca2+ is required for induction of defense responses in the cultured roots on the treatment with Plasmodiophora brassicae resting spores (Takahashi et al., 2006). To evaluate change in [Ca2+]cyt in cultured root cells on contact with the spores, acetoxymethyl ester derivative of Fura-2 (Fura-2/AM) was loaded into the roots. When ionophore A23187 was treated with Ca2+ simultaneously, the Fura-2 fluorescence ratio that represents relative [Ca2+]cyt increased promptly, showing that the Fura-2/AM system is suitable to evaluate [Ca2+]cyt change in cultured root cells in a second range. Applicability of this method for studies on Ca2+ fluctuation against various extracellular stimuli was supported by observations that treatment with mannitol or NaCl also immediately increased the Fura-2 ratio. When the Fura-2/AM loaded roots were treated with resting spores, no [Ca2+]cyt change was observed during 500 second but when treated with spores pre-incubated with germination-enhancing suspension (GES), a slight but reproducible increase in [Ca2+]cyt was observed. We conclude that although further analysis is needed, the Fura-2/AM system will contribute to revealing the Ca2+ involvement in clubroot-resistance response. 我々は以前の研究において、カブ（Brassica rapa L.）幼根から誘導した培養根を用い、根こぶ病菌Plasmodiophora brassicaeに対する初期抵抗反応の誘導にCa2+ が必要であることを示した（Takahashi et al., 2006）。本研究では、根こぶ病抵抗反応におけるカブ培養根の細胞内Ca2+ 濃度（［Ca2+］cyt）の変動を調査するため、Ca2+ の蛍光指示薬Fura-2のアセトキシメチルエステル（Fura-2/AM）をカブ培養根に導入し、蛍光顕微鏡により［Ca2+］cyt を定量的に解析することを試みた。蛍光顕微鏡下で細胞質内にFura-2が導入されたことが確認できたカブ培養根に、カルシウムイオノフォアA23187とCa2+ を同時に処理すると、直ちに［Ca2+］cyt の相対値を示すFura-2蛍光比が上昇したことから、Fura-2/AM を用いた本手法は、培養根における［Ca2+］cyt の変化を観察するのに適していることが示された。さらに［Ca2+］cyt を増加させることが知られている外的刺激として、マンニトール及びNaCl の高濃度処理を行ったところ、同様なFura-2蛍光比の急激な増加が観察され、本手法の汎用性が示された。次に、我々は本来の目的である根こぶ病抵抗反応における［Ca2+］cyt の解析に本手法を応用した。Fura-2/AM を導入した根こぶ病抵抗性カブ培養根にP. brassicae の休眠胞子を処理しても、500秒の測定時間内に［Ca2+］cyt が変化することは無かった。しかし休眠胞子を発芽促進液（GES: germination enhancing suspension）で前処理し、同様の実験を行ったところ、非常にわずかであったものの、再現性ある［Ca2+］cyt の上昇が観察された。根こぶ病に対する初期抵抗反応におけるCa2+ の重要性を立証するためには、今後さらなる解析が必要であるが、その過程において本手法は大きく貢献するものと期待される。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163525", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "高橋, 秀行"}]}, {"nameIdentifiers": [{"nameIdentifier": "163526", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "石川, 寿樹"}]}, {"nameIdentifiers": [{"nameIdentifier": "163527", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "早川, 徹"}]}, {"nameIdentifiers": [{"nameIdentifier": "163528", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "伊藤, 紀美子"}]}, {"nameIdentifiers": [{"nameIdentifier": "163529", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "三ツ井, 敏明"}]}, {"nameIdentifiers": [{"nameIdentifier": "163530", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "堀, 秀隆"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takahashi, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "163519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Toshiki"}], "nameIdentifiers": [{"nameIdentifier": "163520", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "163521", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163522", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163523", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163524", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "1_0009.pdf", "filesize": [{"value": "208.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 208100.0, "url": {"label": "1_0009.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28421/files/1_0009.pdf"}, "version_id": "8d580c25-59e6-4b34-a9cc-8a37e6daeadc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "clubroot resistance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytosolic [Ca2+]", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fura-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "root culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fura-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞内Ca2+ 濃度", "subitem_subject_scheme": "Other"}, {"subitem_subject": "根こぶ病抵抗性", "subitem_subject_scheme": "Other"}, {"subitem_subject": "培養根", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt"}, {"subitem_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1602"], "permalink_uri": "http://hdl.handle.net/10191/5039", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-10-12"}, "publish_date": "2007-10-12", "publish_status": "0", "recid": "28421", "relation": {}, "relation_version_is_last": true, "title": ["Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt"], "weko_shared_id": null}