{"_buckets": {"deposit": "030380d5-a503-4c78-910a-96f62fad480a"}, "_deposit": {"id": "28421", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28421"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028421"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u6839\u3053\u3076\u75c5\u62b5\u6297\u6027\u30ab\u30d6\uff08Brassica rapa L.\uff09\u57f9\u990a\u6839\u3078\u306eFura-2\u306e\u5c0e\u5165\u3068\u7d30\u80de\u5185Ca2+ \u6fc3\u5ea6\u306e\u6e2c\u5b9a"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "66", "bibliographicPageStart": "61", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Our previous study using liquid organ cultured adventitious roots from turnip (Brassica rapa L.) showed that Ca2+ is required for induction of defense responses in the cultured roots on the treatment with Plasmodiophora brassicae resting spores (Takahashi et al., 2006). To evaluate change in [Ca2+]cyt in cultured root cells on contact with the spores, acetoxymethyl ester derivative of Fura-2 (Fura-2/AM) was loaded into the roots. When ionophore A23187 was treated with Ca2+ simultaneously, the Fura-2 fluorescence ratio that represents relative [Ca2+]cyt increased promptly, showing that the Fura-2/AM system is suitable to evaluate [Ca2+]cyt change in cultured root cells in a second range. Applicability of this method for studies on Ca2+ fluctuation against various extracellular stimuli was supported by observations that treatment with mannitol or NaCl also immediately increased the Fura-2 ratio. When the Fura-2/AM loaded roots were treated with resting spores, no [Ca2+]cyt change was observed during 500 second but when treated with spores pre-incubated with germination-enhancing suspension (GES), a slight but reproducible increase in [Ca2+]cyt was observed. We conclude that although further analysis is needed, the Fura-2/AM system will contribute to revealing the Ca2+ involvement in clubroot-resistance response. \u6211\u3005\u306f\u4ee5\u524d\u306e\u7814\u7a76\u306b\u304a\u3044\u3066\u3001\u30ab\u30d6\uff08Brassica rapa L.\uff09\u5e7c\u6839\u304b\u3089\u8a98\u5c0e\u3057\u305f\u57f9\u990a\u6839\u3092\u7528\u3044\u3001\u6839\u3053\u3076\u75c5\u83ccPlasmodiophora brassicae\u306b\u5bfe\u3059\u308b\u521d\u671f\u62b5\u6297\u53cd\u5fdc\u306e\u8a98\u5c0e\u306bCa2+ \u304c\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u305f\uff08Takahashi et al., 2006\uff09\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u6839\u3053\u3076\u75c5\u62b5\u6297\u53cd\u5fdc\u306b\u304a\u3051\u308b\u30ab\u30d6\u57f9\u990a\u6839\u306e\u7d30\u80de\u5185Ca2+ \u6fc3\u5ea6\uff08\uff3bCa2+\uff3dcyt\uff09\u306e\u5909\u52d5\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u3001Ca2+ \u306e\u86cd\u5149\u6307\u793a\u85acFura-2\u306e\u30a2\u30bb\u30c8\u30ad\u30b7\u30e1\u30c1\u30eb\u30a8\u30b9\u30c6\u30eb\uff08Fura-2/AM\uff09\u3092\u30ab\u30d6\u57f9\u990a\u6839\u306b\u5c0e\u5165\u3057\u3001\u86cd\u5149\u9855\u5fae\u93e1\u306b\u3088\u308a\uff3bCa2+\uff3dcyt \u3092\u5b9a\u91cf\u7684\u306b\u89e3\u6790\u3059\u308b\u3053\u3068\u3092\u8a66\u307f\u305f\u3002\u86cd\u5149\u9855\u5fae\u93e1\u4e0b\u3067\u7d30\u80de\u8cea\u5185\u306bFura-2\u304c\u5c0e\u5165\u3055\u308c\u305f\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u305f\u30ab\u30d6\u57f9\u990a\u6839\u306b\u3001\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30ce\u30d5\u30a9\u30a2A23187\u3068Ca2+ \u3092\u540c\u6642\u306b\u51e6\u7406\u3059\u308b\u3068\u3001\u76f4\u3061\u306b\uff3bCa2+\uff3dcyt \u306e\u76f8\u5bfe\u5024\u3092\u793a\u3059Fura-2\u86cd\u5149\u6bd4\u304c\u4e0a\u6607\u3057\u305f\u3053\u3068\u304b\u3089\u3001Fura-2/AM \u3092\u7528\u3044\u305f\u672c\u624b\u6cd5\u306f\u3001\u57f9\u990a\u6839\u306b\u304a\u3051\u308b\uff3bCa2+\uff3dcyt \u306e\u5909\u5316\u3092\u89b3\u5bdf\u3059\u308b\u306e\u306b\u9069\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u3055\u3089\u306b\uff3bCa2+\uff3dcyt \u3092\u5897\u52a0\u3055\u305b\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u5916\u7684\u523a\u6fc0\u3068\u3057\u3066\u3001\u30de\u30f3\u30cb\u30c8\u30fc\u30eb\u53ca\u3073NaCl \u306e\u9ad8\u6fc3\u5ea6\u51e6\u7406\u3092\u884c\u3063\u305f\u3068\u3053\u308d\u3001\u540c\u69d8\u306aFura-2\u86cd\u5149\u6bd4\u306e\u6025\u6fc0\u306a\u5897\u52a0\u304c\u89b3\u5bdf\u3055\u308c\u3001\u672c\u624b\u6cd5\u306e\u6c4e\u7528\u6027\u304c\u793a\u3055\u308c\u305f\u3002\u6b21\u306b\u3001\u6211\u3005\u306f\u672c\u6765\u306e\u76ee\u7684\u3067\u3042\u308b\u6839\u3053\u3076\u75c5\u62b5\u6297\u53cd\u5fdc\u306b\u304a\u3051\u308b\uff3bCa2+\uff3dcyt \u306e\u89e3\u6790\u306b\u672c\u624b\u6cd5\u3092\u5fdc\u7528\u3057\u305f\u3002Fura-2/AM \u3092\u5c0e\u5165\u3057\u305f\u6839\u3053\u3076\u75c5\u62b5\u6297\u6027\u30ab\u30d6\u57f9\u990a\u6839\u306bP. brassicae \u306e\u4f11\u7720\u80de\u5b50\u3092\u51e6\u7406\u3057\u3066\u3082\u3001500\u79d2\u306e\u6e2c\u5b9a\u6642\u9593\u5185\u306b\uff3bCa2+\uff3dcyt \u304c\u5909\u5316\u3059\u308b\u3053\u3068\u306f\u7121\u304b\u3063\u305f\u3002\u3057\u304b\u3057\u4f11\u7720\u80de\u5b50\u3092\u767a\u82bd\u4fc3\u9032\u6db2\uff08GES: germination enhancing suspension\uff09\u3067\u524d\u51e6\u7406\u3057\u3001\u540c\u69d8\u306e\u5b9f\u9a13\u3092\u884c\u3063\u305f\u3068\u3053\u308d\u3001\u975e\u5e38\u306b\u308f\u305a\u304b\u3067\u3042\u3063\u305f\u3082\u306e\u306e\u3001\u518d\u73fe\u6027\u3042\u308b\uff3bCa2+\uff3dcyt \u306e\u4e0a\u6607\u304c\u89b3\u5bdf\u3055\u308c\u305f\u3002\u6839\u3053\u3076\u75c5\u306b\u5bfe\u3059\u308b\u521d\u671f\u62b5\u6297\u53cd\u5fdc\u306b\u304a\u3051\u308bCa2+ 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{"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takahashi, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "163519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Toshiki"}], "nameIdentifiers": [{"nameIdentifier": "163520", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "163521", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163522", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163523", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163524", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "1_0009.pdf", "filesize": [{"value": "208.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 208100.0, "url": {"label": "1_0009.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28421/files/1_0009.pdf"}, "version_id": "8d580c25-59e6-4b34-a9cc-8a37e6daeadc"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "clubroot resistance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytosolic [Ca2+]", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fura-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "root culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fura-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u7d30\u80de\u5185Ca2+ \u6fc3\u5ea6", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u6839\u3053\u3076\u75c5\u62b5\u6297\u6027", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u57f9\u990a\u6839", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt"}, {"subitem_title": "Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1602"], "permalink_uri": "http://hdl.handle.net/10191/5039", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-10-12"}, "publish_date": "2007-10-12", "publish_status": "0", "recid": "28421", "relation": {}, "relation_version_is_last": true, "title": ["Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt"], "weko_shared_id": null}