{"_buckets": {"deposit": "e5b46176-275e-4fed-aa66-28bdd28544d3"}, "_deposit": {"id": "28393", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28393"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028393", "sets": ["456", "1600"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Distributional Changes of Lysosome-Like Bodies in Porcine Embryos during the Early Development"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "39", "bibliographicPageStart": "35", "bibliographicVolumeNumber": "61", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "体外で受精させたブタの卵子および受精後培養して発生させたブタの初期胚について、ライソソーム様小体（LB）の分布が受精および発生に伴ってどのように変化するのかをアクリジンオレンジ染色によって調べた。また、人為的に活性化して発生させた単為発生ブタ胚についても、発生に伴うLB の分布変化を調べ、受精卵子から発生した胚のものと比較した。媒精後あるいは活性化と２倍体化の処置後６ないし32時間の卵子において、LBは、表層を除く細胞質に分布しており、中心部の分布密度は低かった。また、媒精後あるいは活性化と2倍体化の処置後培養して発生させた受精胚と単為発生胚の両者において、LBは、2ないし8細胞期では、割球の核周囲の細胞質に多数分布していたが、桑実胚期と胚盤胞期では、外側の扁平割球あるいは栄養膜細胞では核の側部から上部の細胞質に多数、内側の球形割球あるいは内細胞塊細胞では細胞質全域に多数、それぞれ分布していた。以上の結果から、ブタ卵子のLBは、受精あるいは活性化の処置を施しても卵割するまでは分布変化を起こさないことが確かめられた。また、ブタの受精胚と単為発生胚におけるLBの分布変化は、割球の変形あるいは分化と関係のあることが推察された。", "subitem_description_type": "Abstract"}, {"subitem_description": "Distributional changes of lysosome-like bodies (LBs) in porcine eggs and embryos during the fertilization and early development were observed using the acridine orange staining method, and were compared with those in diploid parthenogenetic embryos derived from the eggs activated and diploidized by the treatment of ethanol and cytochalasin B. At 6 to 32 hrs after insemination or activation, LBs were distributed in the cytoplasm except the cortical region, and its density was lower in the inner cytoplasm of both fertilized and activated eggs. LBs were densely distributed in the perinuclear cytoplasm of blastomeres in fertilized embryos at the 2-cell to 8-cell stages. In fertilized embryos at the stages of morula and blastocyst, LBs were densely distributed throughout the cytoplasm of inner round blastomeres or inner-cell-mass cells, and they were mainly distributed in the cytoplasm of apical and lateral regions of nucleus of outer fl attened blastomeres or trophoblast cells. Such a distribution of LBs in fertilized embryos was similarly observed in parthenogenetic embryos at the 2-cell to blastocyst stages. From these findings, it was confi rmed that a distribution of LBs in matured porcine eggs does not change until the first cleavage. Furthermore, it was suggested that there is a close relationship between distributional changes in LBs and the transformation or differentiation of blastomeres in porcine embryos.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163337", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Yamazaki, Shoko"}]}, {"nameIdentifiers": [{"nameIdentifier": "163338", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimura, Sueo"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "山崎, 祥子"}], "nameIdentifiers": [{"nameIdentifier": "163335", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "新村, 末雄"}], "nameIdentifiers": [{"nameIdentifier": "163336", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "61(1)_35-39.pdf", "filesize": [{"value": "252.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 252900.0, "url": {"label": "61(1)_35-39.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28393/files/61(1)_35-39.pdf"}, "version_id": "f3debbfe-edda-4465-b597-c4b1794546d7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "ブタの卵子と初期胚", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ライソソーム様小体の分布", "subitem_subject_scheme": "Other"}, {"subitem_subject": "アクリジンオレンジ染色", "subitem_subject_scheme": "Other"}, {"subitem_subject": "単為発生胚", "subitem_subject_scheme": "Other"}, {"subitem_subject": "受精と発生", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pig egg and embryo", "subitem_subject_scheme": "Other"}, {"subitem_subject": "distribution of lysosome-like body", "subitem_subject_scheme": "Other"}, {"subitem_subject": "acridine orange staining", "subitem_subject_scheme": "Other"}, {"subitem_subject": "parthenogenetic embryo", "subitem_subject_scheme": "Other"}, {"subitem_subject": "fertilization and development", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ブタ初期胚の発生に伴うライソソーム様小体の分布変化", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ブタ初期胚の発生に伴うライソソーム様小体の分布変化"}, {"subitem_title": "ブタ初期胚の発生に伴うライソソーム様小体の分布変化", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1600"], "permalink_uri": "http://hdl.handle.net/10191/13059", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-02"}, "publish_date": "2010-07-02", "publish_status": "0", "recid": "28393", "relation": {}, "relation_version_is_last": true, "title": ["ブタ初期胚の発生に伴うライソソーム様小体の分布変化"], "weko_shared_id": null}