{"_buckets": {"deposit": "4eb51e3a-f6c4-4a03-b7cc-17f23f60d40a"}, "_deposit": {"id": "28320", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28320"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028320", "sets": ["456", "1592"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "イネSUMO遺伝子の単離および発現と細胞局在性の解析"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "83", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Small ubiquitin-related modifier (SUMO) is a type of ubiquitin-like proteins and regulates various protein functions through post-translational modification. The SUMO precursor proteins are processed by C-terminal cleavage reaction at the end of the di-glycine(GG) motif, and then activated and bind to substrate proteins by a series of enzymatic reaction. We cloned SUMO1-3 genes in rice, and analyzed the transient expression and intracellular localization of the DsRed fusion proteins, DsRed:SUMO1, 2, and 3 in onion epidermal cells by using confocal laser scanning microscopy. The DsRed signals from DsRed:SUMO1 and DsRed:SUMO2 were detected both in nuclei and cytoplasmic location, but not in nucleoli. In the case of DsRed:SUMO3, the DsRed signal was detected mainly in nucleus, and formed sub-nuclear domain like structure. We also tested effect of the GG motif on intracellular localization of SUMO proteins. The GG deletion mutant vectors, pDsRed:SUMO1ΔGG, pDsRed:SUMO2ΔGG, and pDsRed:SUMO3ΔGG were constructed and transiently expressed in onion epidermal cells. Result showed that the deletion mutation of GG motif suppressed the accumulation of the DsRed:SUMOΔGG proteins in the nucleus. These results indicated that the C-terminal processing of OsSUMO precursor proteins are necessary for OsSUMO localization to nucleus in onion epidermal cells.", "subitem_description_type": "Abstract"}, {"subitem_description": "Small-ubiquitin related modifier (SUMO)はユビキチン様タンパク質の一種であり、翻訳後修飾により多様なタンパク質の機能を調節する。SUMO 前駆体タンパク質はＣ末側に存在するジグリシン（GG）モチーフの末端でプロセシングされ、一連の酵素反応により活性化され基質タンパク質に結合する。私たちはイネSUMO1-3遺伝子を単離し、DsRed 融合タンパク質であるDsRed:SUMO1, ２、および３をタマネギ表皮細胞において一過的に発現させ、その細胞局在性を共焦点レーザー顕微鏡により解析した。DsRed:SUMO1およびDsRed:SUMO2のシグナルは核と細胞質にも分布したが、核小体には観察されなかった。DsRed:SUMO3の場合は、主に核に局在し、そのシグナルは細胞核ドメイン様構造を形成した。私たちはまた、GGモチーフ欠失変異細胞内局在性に関わる影響をテストした。GG モチーフ欠失変異を持つベクター、pDsRed:SUMO1ΔGG、pDsRed:SUMO2Δ GG、およびpDsRed:SUMO3ΔGG を構築し、タマネギ表皮細胞において発現させた。GG モチーフの欠失変異はDsRed:SUMO Δ GG タンパク質の核への集積を抑制した。これらの結果から、OsSUMO 前駆体タンパク質のＣ末端のプロセシングがタマネギ表皮細胞におけるOsSUMO タンパク質の核への局在性に必要であることが示唆された。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162798", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ikarashi, Yuta"}]}, {"nameIdentifiers": [{"nameIdentifier": "162799", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Noguchi, Natsuki"}]}, {"nameIdentifiers": [{"nameIdentifier": "162800", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Attia, Kotb"}]}, {"nameIdentifiers": [{"nameIdentifier": "162801", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kitajima-Koga, Aya"}]}, {"nameIdentifiers": [{"nameIdentifier": "162802", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Mitsui, Toshiaki"}]}, {"nameIdentifiers": [{"nameIdentifier": "162803", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Itoh, Kimiko"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "五十嵐, 雄太"}], "nameIdentifiers": [{"nameIdentifier": "162792", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "野口, 夏希"}], "nameIdentifiers": [{"nameIdentifier": "162793", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "アティア, コティブ"}], "nameIdentifiers": [{"nameIdentifier": "162794", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "北島-古賀, 彩"}], "nameIdentifiers": [{"nameIdentifier": "162795", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "三ツ井, 敏明"}], "nameIdentifiers": [{"nameIdentifier": "162796", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "伊藤, 紀美子"}], "nameIdentifiers": [{"nameIdentifier": "162797", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(1)_77-83.pdf", "filesize": [{"value": "852.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 852900.0, "url": {"label": "65(1)_77-83.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28320/files/65(1)_77-83.pdf"}, "version_id": "78173f6e-37f4-48ab-81ff-b596377c480b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "SUMO", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "onion epidermal cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GG motif", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DsRed", "subitem_subject_scheme": "Other"}, {"subitem_subject": "イネ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "タマネギ表皮細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GGモチーフ", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cloning, expression, and intracellular localization of rice SUMO genes.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Cloning, expression, and intracellular localization of rice SUMO genes."}, {"subitem_title": "Cloning, expression, and intracellular localization of rice SUMO genes.", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1592"], "permalink_uri": "http://hdl.handle.net/10191/22569", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28320", "relation": {}, "relation_version_is_last": true, "title": ["Cloning, expression, and intracellular localization of rice SUMO genes."], "weko_shared_id": null}