{"_buckets": {"deposit": "4eb51e3a-f6c4-4a03-b7cc-17f23f60d40a"}, "_deposit": {"id": "28320", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28320"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028320"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a4\u30cdSUMO\u907a\u4f1d\u5b50\u306e\u5358\u96e2\u304a\u3088\u3073\u767a\u73fe\u3068\u7d30\u80de\u5c40\u5728\u6027\u306e\u89e3\u6790"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "83", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Small ubiquitin-related modifier (SUMO) is a type of ubiquitin-like proteins and regulates various protein functions through post-translational modification. The SUMO precursor proteins are processed by C-terminal cleavage reaction at the end of the di-glycine(GG) motif, and then activated and bind to substrate proteins by a series of enzymatic reaction. We cloned SUMO1-3 genes in rice, and analyzed the transient expression and intracellular localization of the DsRed fusion proteins, DsRed:SUMO1, 2, and 3 in onion epidermal cells by using confocal laser scanning microscopy. The DsRed signals from DsRed:SUMO1 and DsRed:SUMO2 were detected both in nuclei and cytoplasmic location, but not in nucleoli. In the case of DsRed:SUMO3, the DsRed signal was detected mainly in nucleus, and formed sub-nuclear domain like structure. We also tested effect of the GG motif on intracellular localization of SUMO proteins. The GG deletion mutant vectors, pDsRed:SUMO1\u0394GG, pDsRed:SUMO2\u0394GG, and pDsRed:SUMO3\u0394GG were constructed and transiently expressed in onion epidermal cells. Result showed that the deletion mutation of GG motif suppressed the accumulation of the DsRed:SUMO\u0394GG proteins in the nucleus. These results indicated that the C-terminal processing of OsSUMO precursor proteins are necessary for OsSUMO localization to nucleus in onion epidermal cells.", "subitem_description_type": "Abstract"}, {"subitem_description": "Small-ubiquitin related modifier (SUMO)\u306f\u30e6\u30d3\u30ad\u30c1\u30f3\u69d8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e00\u7a2e\u3067\u3042\u308a\u3001\u7ffb\u8a33\u5f8c\u4fee\u98fe\u306b\u3088\u308a\u591a\u69d8\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u8abf\u7bc0\u3059\u308b\u3002SUMO 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