WEKO3
AND
アイテム
{"_buckets": {"deposit": "4eb51e3a-f6c4-4a03-b7cc-17f23f60d40a"}, "_deposit": {"id": "28320", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28320"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028320"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a4\u30cdSUMO\u907a\u4f1d\u5b50\u306e\u5358\u96e2\u304a\u3088\u3073\u767a\u73fe\u3068\u7d30\u80de\u5c40\u5728\u6027\u306e\u89e3\u6790"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "83", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Small ubiquitin-related modifier (SUMO) is a type of ubiquitin-like proteins and regulates various protein functions through post-translational modification. The SUMO precursor proteins are processed by C-terminal cleavage reaction at the end of the di-glycine(GG) motif, and then activated and bind to substrate proteins by a series of enzymatic reaction. We cloned SUMO1-3 genes in rice, and analyzed the transient expression and intracellular localization of the DsRed fusion proteins, DsRed:SUMO1, 2, and 3 in onion epidermal cells by using confocal laser scanning microscopy. The DsRed signals from DsRed:SUMO1 and DsRed:SUMO2 were detected both in nuclei and cytoplasmic location, but not in nucleoli. In the case of DsRed:SUMO3, the DsRed signal was detected mainly in nucleus, and formed sub-nuclear domain like structure. We also tested effect of the GG motif on intracellular localization of SUMO proteins. The GG deletion mutant vectors, pDsRed:SUMO1\u0394GG, pDsRed:SUMO2\u0394GG, and pDsRed:SUMO3\u0394GG were constructed and transiently expressed in onion epidermal cells. Result showed that the deletion mutation of GG motif suppressed the accumulation of the DsRed:SUMO\u0394GG proteins in the nucleus. These results indicated that the C-terminal processing of OsSUMO precursor proteins are necessary for OsSUMO localization to nucleus in onion epidermal cells.", "subitem_description_type": "Abstract"}, {"subitem_description": "Small-ubiquitin related modifier (SUMO)\u306f\u30e6\u30d3\u30ad\u30c1\u30f3\u69d8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e00\u7a2e\u3067\u3042\u308a\u3001\u7ffb\u8a33\u5f8c\u4fee\u98fe\u306b\u3088\u308a\u591a\u69d8\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u8abf\u7bc0\u3059\u308b\u3002SUMO \u524d\u99c6\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u306f\uff23\u672b\u5074\u306b\u5b58\u5728\u3059\u308b\u30b8\u30b0\u30ea\u30b7\u30f3\uff08GG\uff09\u30e2\u30c1\u30fc\u30d5\u306e\u672b\u7aef\u3067\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u3055\u308c\u3001\u4e00\u9023\u306e\u9175\u7d20\u53cd\u5fdc\u306b\u3088\u308a\u6d3b\u6027\u5316\u3055\u308c\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3059\u308b\u3002\u79c1\u305f\u3061\u306f\u30a4\u30cdSUMO1-3\u907a\u4f1d\u5b50\u3092\u5358\u96e2\u3057\u3001DsRed \u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308bDsRed:SUMO1, \uff12\u3001\u304a\u3088\u3073\uff13\u3092\u30bf\u30de\u30cd\u30ae\u8868\u76ae\u7d30\u80de\u306b\u304a\u3044\u3066\u4e00\u904e\u7684\u306b\u767a\u73fe\u3055\u305b\u3001\u305d\u306e\u7d30\u80de\u5c40\u5728\u6027\u3092\u5171\u7126\u70b9\u30ec\u30fc\u30b6\u30fc\u9855\u5fae\u93e1\u306b\u3088\u308a\u89e3\u6790\u3057\u305f\u3002DsRed:SUMO1\u304a\u3088\u3073DsRed:SUMO2\u306e\u30b7\u30b0\u30ca\u30eb\u306f\u6838\u3068\u7d30\u80de\u8cea\u306b\u3082\u5206\u5e03\u3057\u305f\u304c\u3001\u6838\u5c0f\u4f53\u306b\u306f\u89b3\u5bdf\u3055\u308c\u306a\u304b\u3063\u305f\u3002DsRed:SUMO3\u306e\u5834\u5408\u306f\u3001\u4e3b\u306b\u6838\u306b\u5c40\u5728\u3057\u3001\u305d\u306e\u30b7\u30b0\u30ca\u30eb\u306f\u7d30\u80de\u6838\u30c9\u30e1\u30a4\u30f3\u69d8\u69cb\u9020\u3092\u5f62\u6210\u3057\u305f\u3002\u79c1\u305f\u3061\u306f\u307e\u305f\u3001GG\u30e2\u30c1\u30fc\u30d5\u6b20\u5931\u5909\u7570\u7d30\u80de\u5185\u5c40\u5728\u6027\u306b\u95a2\u308f\u308b\u5f71\u97ff\u3092\u30c6\u30b9\u30c8\u3057\u305f\u3002GG \u30e2\u30c1\u30fc\u30d5\u6b20\u5931\u5909\u7570\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc\u3001pDsRed:SUMO1\u0394GG\u3001pDsRed:SUMO2\u0394 GG\u3001\u304a\u3088\u3073pDsRed:SUMO3\u0394GG \u3092\u69cb\u7bc9\u3057\u3001\u30bf\u30de\u30cd\u30ae\u8868\u76ae\u7d30\u80de\u306b\u304a\u3044\u3066\u767a\u73fe\u3055\u305b\u305f\u3002GG \u30e2\u30c1\u30fc\u30d5\u306e\u6b20\u5931\u5909\u7570\u306fDsRed:SUMO \u0394 GG \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6838\u3078\u306e\u96c6\u7a4d\u3092\u6291\u5236\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\u3001OsSUMO \u524d\u99c6\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\uff23\u672b\u7aef\u306e\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u304c\u30bf\u30de\u30cd\u30ae\u8868\u76ae\u7d30\u80de\u306b\u304a\u3051\u308bOsSUMO \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6838\u3078\u306e\u5c40\u5728\u6027\u306b\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162798", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ikarashi, Yuta"}]}, {"nameIdentifiers": [{"nameIdentifier": "162799", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Noguchi, Natsuki"}]}, {"nameIdentifiers": [{"nameIdentifier": "162800", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Attia, Kotb"}]}, {"nameIdentifiers": [{"nameIdentifier": "162801", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kitajima-Koga, Aya"}]}, {"nameIdentifiers": [{"nameIdentifier": "162802", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Mitsui, Toshiaki"}]}, {"nameIdentifiers": [{"nameIdentifier": "162803", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Itoh, Kimiko"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u4e94\u5341\u5d50, \u96c4\u592a"}], "nameIdentifiers": [{"nameIdentifier": "162792", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u91ce\u53e3, \u590f\u5e0c"}], "nameIdentifiers": [{"nameIdentifier": "162793", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u30a2\u30c6\u30a3\u30a2, \u30b3\u30c6\u30a3\u30d6"}], "nameIdentifiers": [{"nameIdentifier": "162794", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5317\u5cf6-\u53e4\u8cc0, \u5f69"}], "nameIdentifiers": [{"nameIdentifier": "162795", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u4e09\u30c4\u4e95, \u654f\u660e"}], "nameIdentifiers": [{"nameIdentifier": "162796", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u4f0a\u85e4, \u7d00\u7f8e\u5b50"}], "nameIdentifiers": [{"nameIdentifier": "162797", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(1)_77-83.pdf", "filesize": [{"value": "852.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 852900.0, "url": {"label": "65(1)_77-83.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28320/files/65(1)_77-83.pdf"}, "version_id": "78173f6e-37f4-48ab-81ff-b596377c480b"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "SUMO", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "onion epidermal cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GG motif", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DsRed", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30a4\u30cd", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30bf\u30de\u30cd\u30ae\u8868\u76ae\u7d30\u80de", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GG\u30e2\u30c1\u30fc\u30d5", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cloning, expression, and intracellular localization of rice SUMO genes.", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Cloning, expression, and intracellular localization of rice SUMO genes."}, {"subitem_title": "Cloning, expression, and intracellular localization of rice SUMO genes.", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1592"], "permalink_uri": "http://hdl.handle.net/10191/22569", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28320", "relation": {}, "relation_version_is_last": true, "title": ["Cloning, expression, and intracellular localization of rice SUMO genes."], "weko_shared_id": null}
Cloning, expression, and intracellular localization of rice SUMO genes.
http://hdl.handle.net/10191/22569
abacf1b1-fe1c-4fb3-960c-f8c7220b5f5b
名前 / ファイル | ライセンス | アクション | |
---|---|---|---|
![]() |
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-05-29 | |||||
タイトル | ||||||
タイトル | Cloning, expression, and intracellular localization of rice SUMO genes. | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cloning, expression, and intracellular localization of rice SUMO genes. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SUMO | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | onion epidermal cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GG motif | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DsRed | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | イネ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | タマネギ表皮細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GGモチーフ | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | イネSUMO遺伝子の単離および発現と細胞局在性の解析 | |||||
著者 |
五十嵐, 雄太
× 五十嵐, 雄太× 野口, 夏希× アティア, コティブ× 北島-古賀, 彩× 三ツ井, 敏明× 伊藤, 紀美子 |
|||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162798 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Ikarashi, Yuta | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162799 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Noguchi, Natsuki | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162800 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Attia, Kotb | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162801 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Kitajima-Koga, Aya | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162802 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Mitsui, Toshiaki | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 162803 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Itoh, Kimiko | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Small ubiquitin-related modifier (SUMO) is a type of ubiquitin-like proteins and regulates various protein functions through post-translational modification. The SUMO precursor proteins are processed by C-terminal cleavage reaction at the end of the di-glycine(GG) motif, and then activated and bind to substrate proteins by a series of enzymatic reaction. We cloned SUMO1-3 genes in rice, and analyzed the transient expression and intracellular localization of the DsRed fusion proteins, DsRed:SUMO1, 2, and 3 in onion epidermal cells by using confocal laser scanning microscopy. The DsRed signals from DsRed:SUMO1 and DsRed:SUMO2 were detected both in nuclei and cytoplasmic location, but not in nucleoli. In the case of DsRed:SUMO3, the DsRed signal was detected mainly in nucleus, and formed sub-nuclear domain like structure. We also tested effect of the GG motif on intracellular localization of SUMO proteins. The GG deletion mutant vectors, pDsRed:SUMO1ΔGG, pDsRed:SUMO2ΔGG, and pDsRed:SUMO3ΔGG were constructed and transiently expressed in onion epidermal cells. Result showed that the deletion mutation of GG motif suppressed the accumulation of the DsRed:SUMOΔGG proteins in the nucleus. These results indicated that the C-terminal processing of OsSUMO precursor proteins are necessary for OsSUMO localization to nucleus in onion epidermal cells. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Small-ubiquitin related modifier (SUMO)はユビキチン様タンパク質の一種であり、翻訳後修飾により多様なタンパク質の機能を調節する。SUMO 前駆体タンパク質はC末側に存在するジグリシン(GG)モチーフの末端でプロセシングされ、一連の酵素反応により活性化され基質タンパク質に結合する。私たちはイネSUMO1-3遺伝子を単離し、DsRed 融合タンパク質であるDsRed:SUMO1, 2、および3をタマネギ表皮細胞において一過的に発現させ、その細胞局在性を共焦点レーザー顕微鏡により解析した。DsRed:SUMO1およびDsRed:SUMO2のシグナルは核と細胞質にも分布したが、核小体には観察されなかった。DsRed:SUMO3の場合は、主に核に局在し、そのシグナルは細胞核ドメイン様構造を形成した。私たちはまた、GGモチーフ欠失変異細胞内局在性に関わる影響をテストした。GG モチーフ欠失変異を持つベクター、pDsRed:SUMO1ΔGG、pDsRed:SUMO2Δ GG、およびpDsRed:SUMO3ΔGG を構築し、タマネギ表皮細胞において発現させた。GG モチーフの欠失変異はDsRed:SUMO Δ GG タンパク質の核への集積を抑制した。これらの結果から、OsSUMO 前駆体タンパク質のC末端のプロセシングがタマネギ表皮細胞におけるOsSUMO タンパク質の核への局在性に必要であることが示唆された。 | |||||
書誌情報 |
新潟大学農学部研究報告 en : 新潟大学農学部研究報告 巻 65, 号 1, p. 77-83, 発行日 2012-09 |
|||||
出版者 | ||||||
出版者 | 新潟大学農学部 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03858634 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00183393 | |||||
著者版フラグ | ||||||
値 | publisher |